RPL5 Polyclonal Antibody
- Size: 100ul
- Application: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
- Reactivity: Human, Rat
- Predicted Reactivity: Mouse, Dog, Cow, Sheep, Pig, Horse, Rabbit
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Datasheet
Tech Support
IMAGES
Paraformaldehyde-fixed, paraffin embedded Human gastric carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with RPL5 Polyclonal Antibody, Unconjugated (bs-6573R) at 1:200 overnight at 4°C, DAB staining.
Rat brain lysates probed with Anti RPL5 Polyclonal Antibody, Unconjugated (bs-6573R) at 1:200 overnight at 4°C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37°C. Predicted band 34kD. Observed band size: 34kD
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with RPL5 Polyclonal Antibody, Unconjugated (bs-6573R) at 1:200 overnight at 4°C, DAB staining.
PRODUCT DETAILS
SPECIFICATIONS |
|
|---|---|
|
PRODUCT NAME |
RPL5 Polyclonal Antibody |
|
CONJUGATION |
Unconjugated |
|
HOST |
Rabbit |
| SOURCE |
KLH conjugated synthetic peptide derived from human RPL5 |
| IMMUNOGEN RANGE |
121-220/294 |
| CLONALITY |
Polyclonal |
| ISOTYPE |
IgG |
| CONCENTRATION |
1ug/ul |
| PURIFICATION |
Purified by Protein A. |
| STORAGE BUFFER |
Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. |
| STORAGE CONDITION |
Store at -20°C for 12 months.. |
TARGET |
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|---|---|
| GENE ID |
|
| SUBCELLULAR LOCATION |
Cytoplasm, Nucleus |
| SYNONYMS |
L5; DBA6; MSTP030; PPP1R135; 60S ribosomal protein L5; RPL5 |
| BACKGROUND |
Required for rRNA maturation and formation of the 60S ribosomal subunits. This protein binds 5S RNA. |
| APPLICATION DILUTION |
WB(1:300-1000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200) |
