PRODUCT NAME

Rad17 Polyclonal Antibody, Cy5 Conjugated

CONJUGATION

Cy5

HOST

Rabbit

SOURCE

KLH conjugated synthetic peptide derived from human Rad17

IMMUNOGEN RANGE

101-230/681

CLONALITY

Polyclonal

ISOTYPE

IgG

CONCENTRATION

1ug/ul

PURIFICATION

Purified by Protein A.

STORAGE BUFFER

Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide.

STORAGE CONDITION

Store at 4°C for 12 months.

GENE ID

5884

SUBCELLULAR LOCATION

Nucleus

SYNONYMS

RFC; CCYC; Cell cycle checkpoint protein; Cell cycle checkpoint protein RAD17; HRAD 17; hRad17; R24L; Rad 17; Rad 24; RAD1 S. pombe homolog; RAD17; RAD17 homolog; Rad17 like protein; RAD17_HUMAN; RAD17Sp; Rad24; RF C activator 1 homolog; RF C/activator 1 homolog; RF-C/activator 1 homolog.

BACKGROUND

The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad17, a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein shares strong similarity with DNA replication factor C (RFC), and can form a complex with RFCs. This protein binds to chromatin prior to DNA damage and is phosphorylated by the checkpoint kinase ATR following damage. This protein recruits the RAD1-RAD9-HUS1 checkpoint protein complex onto chromatin after DNA damage, which may be required for its phosphorylation. The phosphorylation of this protein is required for the DNA-damage-induced cell cycle G2 arrest, and is thought to be a critical early event during checkpoint signaling in DNA-damaged cells. Multiple alternatively spliced transcript variants of this gene, which encode four distinct protein isoforms, have been reported. Two pseudogenes, located on chromosomes 7 and 13, have been identified. [provided by RefSeq, Jul 2013].

APPLICATION DILUTION

IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)