MDM2 Monoclonal Antibody
- Size: 100ul
- Application: WB
- Reactivity:
- Predicted Reactivity: Human
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Datasheet
Tech Support
IMAGES
Lane 1: MCF-7 cell lysates probed with MDM2 Monoclonal Antibody, Unconjugated (bsm-51662M) at 1:4000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.
PRODUCT DETAILS
SPECIFICATIONS |
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PRODUCT NAME |
MDM2 Monoclonal Antibody |
|
CONJUGATION |
Unconjugated |
|
HOST |
Mouse |
| SOURCE |
Recombinant human MDM2 between 196-435 amino acids. |
| IMMUNOGEN RANGE |
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| CLONALITY |
Monoclonal |
| ISOTYPE |
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| CONCENTRATION |
1ug/ul |
| PURIFICATION |
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| STORAGE BUFFER |
0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| STORAGE CONDITION |
Shipped at 4_. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
TARGET |
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| GENE ID |
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| SUBCELLULAR LOCATION |
Cytoplasm, Nucleus |
| SYNONYMS |
Double minute 2 protein; Hdm 2; HDM2; MDM 2; Mdm2 transformed 3T3 cell double minute 2 p53 binding protein (mouse) binding protein 104kDa; MDM2BP; Mouse Double Minute 2; MTBP; Murine Double Minute Chromosome 2; Oncoprotein Mdm2; p53 Binding Protein Mdm2; Ubiquitin protein ligase E3 Mdm2; MDM2_HUMAN; E3 ubiquitin-protein ligase Mdm2. |
| BACKGROUND |
E3 ubiquitin-protein ligase that mediates ubiquitination of p53/TP53, leading to its degradation by the proteasome. Inhibits p53/TP53- and p73/TP73-mediated cell cycle arrest and apoptosis by binding its transcriptional activation domain. Also acts as an ubiquitin ligase E3 toward itself and ARRB1. Permits the nuclear export of p53/TP53. Promotes proteasome-dependent ubiquitin-independent degradation of retinoblastoma RB1 protein. Inhibits DAXX-mediated apoptosis by inducing its ubiquitination and degradation. Component of the TRIM28/KAP1-MDM2-p53/TP53 complex involved in stabilizing p53/TP53. Also component of the TRIM28/KAP1-ERBB4-MDM2 complex which links growth factor and DNA damage response pathways. |
| APPLICATION DILUTION |
WB(1:300-1000) |