MBD2 Monoclonal Antibody
- Size: 100ul
- Application: WB, IHC-P
- Reactivity:
- Predicted Reactivity: Human
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Datasheet
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IMAGES
Paraformaldehyde-fixed, paraffin embedded Human colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with MBD2 Monoclonal Antibody, Unconjugated (bsm-51671M) at 1:25 overnight at 4°C, DAB staining.
PRODUCT DETAILS
SPECIFICATIONS |
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|---|---|
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PRODUCT NAME |
MBD2 Monoclonal Antibody |
|
CONJUGATION |
Unconjugated |
|
HOST |
Mouse |
| SOURCE |
Recombinant human MBD2 between 10-228 amino acids. |
| IMMUNOGEN RANGE |
|
| CLONALITY |
Monoclonal |
| ISOTYPE |
|
| CONCENTRATION |
1ug/ul |
| PURIFICATION |
|
| STORAGE BUFFER |
0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| STORAGE CONDITION |
Shipped at 4_. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
TARGET |
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|---|---|
| GENE ID |
|
| SUBCELLULAR LOCATION |
Nucleus |
| SYNONYMS |
Demethylase; DMTase; MBD 2; MBD2_HUMAN; MBD2a; Methyl CpG binding domain protein 2; Methyl CpG binding protein MBD2; Methyl-CpG-binding domain protein 2; Methyl-CpG-binding protein MBD2; NY CO 41. |
| BACKGROUND |
Binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. Binds hemimethylated DNA as well. Recruits histone deacetylases and DNA methyltransferases. Acts as transcriptional repressor and plays a role in gene silencing. Functions as a scaffold protein, targeting GATAD2A and GATAD2B to chromatin to promote repression. May enhance the activation of some unmethylated cAMP-responsive promoters. |
| APPLICATION DILUTION |
WB(1:300-1000), IHC-P(1:200-400) |
