PRODUCT NAME

LATS1 (Thr1079) Polyclonal Antibody, ALEXA FLUOR¨ 350 Conjugated

CONJUGATION

ALEXA FLUOR¨ 350

HOST

Rabbit

SOURCE

KLH conjugated synthetic phosphopeptide derived from human LATS1 around the phosphorylation site of Thr1079

IMMUNOGEN RANGE

CLONALITY

Polyclonal

ISOTYPE

IgG

CONCENTRATION

1ug/ul

PURIFICATION

Purified by Protein A.

STORAGE BUFFER

Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide.

STORAGE CONDITION

Store at 4°C for 12 months.

GENE ID

9113

SUBCELLULAR LOCATION

Cytoplasm

SYNONYMS

wts; WARTS; Serine/threonine-protein kinase LATS1; Large tumor suppressor homolog 1; WARTS protein kinase; h-warts; LATS1

BACKGROUND

Negative regulator of YAP1 in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS1 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. Acts as a tumor suppressor which plays a critical role in maintenance of ploidy through its actions in both mitotic progression and the G1 tetraploidy checkpoint. Negatively regulates G2/M transition by down-regulating CDK1 kinase activity. Involved in the control of p53 expression. Affects cytokinesis by regulating actin polymerization through negative modulation of LIMK1. May also play a role in endocrine function.

APPLICATION DILUTION

IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)