IRS1 (Tyr1179) Polyclonal Antibody
- Size: 100ul
- Application: WB, ELISA, FCM, IHC-P, IHC-F, IF(ICC), IF
- Reactivity: Human, Mouse, Rat
- Predicted Reactivity:
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Datasheet
Tech Support
IMAGES
HepG2 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20°C and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with IRS1 (Tyr1179) Polyclonal Antibody(bs-8709R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
PRODUCT DETAILS
SPECIFICATIONS |
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|---|---|
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PRODUCT NAME |
IRS1 (Tyr1179) Polyclonal Antibody |
|
CONJUGATION |
Unconjugated |
|
HOST |
Rabbit |
| SOURCE |
KLH conjugated synthesised phosphopeptide derived from human IRS1 around the phosphorylation site of Ser1179. |
| IMMUNOGEN RANGE |
|
| CLONALITY |
Polyclonal |
| ISOTYPE |
IgG |
| CONCENTRATION |
1ug/ul |
| PURIFICATION |
Purified by Protein A. |
| STORAGE BUFFER |
Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4 |
| STORAGE CONDITION |
Store at -20°C for 12 months.. |
TARGET |
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|---|---|
| GENE ID |
|
| SUBCELLULAR LOCATION |
Cell membrane |
| SYNONYMS |
IRS1 (phospho S1179); p-IRS1 (phospho S1179); p-IRS1; IRS1(phospho-Ser1179); HIRS 1; HIRS1; Insulin Receptor Substrate 1; IRS-1; IRS 1; IRS1; OTTHUMP00000164234; IRS1_HUMAN. |
| BACKGROUND |
May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit. |
| APPLICATION DILUTION |
WB(1:300-1000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(ICC)(1:50-200), IF() |
