DYKDDDDK (10A2) Monoclonal Antibody
- Size: 100ul
- Application: WB, FCM, IF(ICC)
- Reactivity: Human, All
- Predicted Reactivity:
-
Datasheet
Tech Support
IMAGES
Flow cytometric analysis of 293T cells transfected with FLAG-PQBP1 with DYKDDDDK (10A2) Monoclonal Antibody (bsm-58004R) at a 1:100 dilution followed by secondary antibody incubation (orange), compared to primary antibody only without secondary antibody (blue), and secondary antibody only staining (red).
293T transfected with DYKDDDDK protein, Lane 1: DYKDDDDK -protein 1 at 25ug; Lane 2: DYKDDDDK-protein 2 at 2ug; lysate was probed with DYKDDDDK (10A2) Monoclonal Antibody, Unconjugated (bsm-58004R) at 1 µg/ml dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.
IF(ICC) staining with DYKDDDDK (10A2) Monoclonal Antibody (bsm-58004R) at 1:200 in 293T cells transfected with FLAG-GAPDH (green). The nuclear counterstain is DAPI (blue).
PRODUCT DETAILS
SPECIFICATIONS |
|
|---|---|
|
PRODUCT NAME |
DYKDDDDK (10A2) Monoclonal Antibody |
|
CONJUGATION |
Unconjugated |
|
HOST |
Rabbit |
| SOURCE |
DYKDDDDK peptide |
| IMMUNOGEN RANGE |
|
| CLONALITY |
Monoclonal |
| ISOTYPE |
IgG |
| CONCENTRATION |
Not Determined |
| PURIFICATION |
Purified by Protein A. |
| STORAGE BUFFER |
Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. |
| STORAGE CONDITION |
Store for 1 year at -20°C from date of shipment. Store for 1 year at -20°C from date of shipment. |
TARGET |
|
|---|---|
| GENE ID |
|
| SUBCELLULAR LOCATION |
|
| SYNONYMS |
|
| BACKGROUND |
DYKDDDDK polypeptide tag (i.e. FLAG tag, currently owned by Sigma) is a common protein tag added to recombinant proteins. DYKDDDDK was originally used by Munro and Pelham in 1984. This artificially designed tag facilitates the identification and purification of recombinant proteins. The hydrophilic nature of DYKDDDDK make it less likely to denature or inactivate proteins to which it is fused. Moreover, N-terminal FLAG tags can be readily cleaved from proteins with enterokinase. It is one of the most widely used protein tags. |
| APPLICATION DILUTION |
WB(1:300-1000), FCM(1:20-100), IF(ICC)(1:50-200) |