CD27 Monoclonal Antibody
- Size: 100ul
- Application: WB, IHC-P
- Reactivity: Human, Mouse, Rat
- Predicted Reactivity:
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Datasheet
Tech Support
IMAGES
Lane 1: Mouse Spleen lysates; Lane 2: Mouse Lymph node lysates; Lane 3: Mouse Thymus lysates; Lane 4: Mouse Plasma lysates probed with CD27 (3C3) Monoclonal Antibody, Unconjugated (bsm-54318R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.
Paraformaldehyde-fixed, paraffin embedded Mouse lymph node; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with CD27 (3C3) Monoclonal Antibody, Unconjugated (bsm-54318R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with CD27 (3C3) Monoclonal Antibody, Unconjugated (bsm-54318R) at 1:200 overnight at 4°C, DAB staining.
PRODUCT DETAILS
SPECIFICATIONS |
|
|---|---|
|
PRODUCT NAME |
CD27 Monoclonal Antibody |
|
CONJUGATION |
Unconjugated |
|
HOST |
Rabbit |
| SOURCE |
KLH conjugated synthetic peptide derived from human CD27 |
| IMMUNOGEN RANGE |
|
| CLONALITY |
Monoclonal |
| ISOTYPE |
IgG |
| CONCENTRATION |
1ug/ul |
| PURIFICATION |
Purified by Protein A. |
| STORAGE BUFFER |
Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| STORAGE CONDITION |
Store at -20°C for 12 months.. |
TARGET |
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|---|---|
| GENE ID |
|
| SUBCELLULAR LOCATION |
Cytomembrane |
| SYNONYMS |
CD 27; Cd27; CD27 antigen; CD27 molecule; CD27_HUMAN; CD27L receptor; LPFS2; MGC20393; OTTHUMP00000238557; S152; T cell activation antigen CD27; T cell antivation antigen S152; T-cell activation antigen CD27; T14; TNFRSF 7; TNFRSF7; TNFSF7; Tp 55; Tp55; Tumor necrosis factor receptor superfamily member 7. |
| BACKGROUND |
Receptor for CD70/CD27L. May play a role in survival of activated T-cells. May play a role in apoptosis through association with SIVA1. |
| APPLICATION DILUTION |
WB(1:300-1000), IHC-P(1:200-400) |
