Paraformaldehyde-fixed, paraffin embedded Human liver cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Calpain small subunit 1 Monoclonal Antibody, Unconjugated (bsm-54658R) at 1:50 for 30 minutes at room temperature, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat uterus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Calpain small subunit 1 Monoclonal Antibody, Unconjugated (bsm-54658R) at 1:200 for 30 minutes at room temperature, DAB staining.
Flow cytometric analysis of Calpain small subunit 1 was done on A549 cells. The cells were fixed, permeabilized and stained with the primary antibody (bsm-54658R, 1/50) (red). After incubation of the primary antibody at room temperature for an hour,
Paraformaldehyde-fixed, paraffin embedded Mouse liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Calpain small subunit 1 Monoclonal Antibody, Unconjugated (bsm-54658R) at 1:50 for 30 minutes at room temperature, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Calpain small subunit 1 Monoclonal Antibody, Unconjugated (bsm-54658R) at 1:50 for 30 minutes at room temperature, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Calpain small subunit 1 Monoclonal Antibody, Unconjugated (bsm-54658R) at 1:50 for 30 minutes at room temperature, DAB staining.
PRODUCT DETAILS
SPECIFICATIONS
PRODUCT NAME
Calpain small subunit 1 Monoclonal Antibody
CONJUGATION
Unconjugated
HOST
Rabbit
SOURCE
Recombinant protein within N-terminal human Calpain small subunit 1.
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