Calpain small subunit 1 Monoclonal Antibody
- Size: 100ul
- Application: WB, FCM, IHC-P, IHC
- Reactivity: Human, Mouse, Rat
- Predicted Reactivity:
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Datasheet
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IMAGES
Paraformaldehyde-fixed, paraffin embedded Human liver cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Calpain small subunit 1 Monoclonal Antibody, Unconjugated (bsm-54658R) at 1:50 for 30 minutes at room temperature, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat uterus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Calpain small subunit 1 Monoclonal Antibody, Unconjugated (bsm-54658R) at 1:200 for 30 minutes at room temperature, DAB staining.
Flow cytometric analysis of Calpain small subunit 1 was done on A549 cells. The cells were fixed, permeabilized and stained with the primary antibody (bsm-54658R, 1/50) (red). After incubation of the primary antibody at room temperature for an hour,
Paraformaldehyde-fixed, paraffin embedded Mouse liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Calpain small subunit 1 Monoclonal Antibody, Unconjugated (bsm-54658R) at 1:50 for 30 minutes at room temperature, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Calpain small subunit 1 Monoclonal Antibody, Unconjugated (bsm-54658R) at 1:50 for 30 minutes at room temperature, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Calpain small subunit 1 Monoclonal Antibody, Unconjugated (bsm-54658R) at 1:50 for 30 minutes at room temperature, DAB staining.
PRODUCT DETAILS
SPECIFICATIONS |
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|---|---|
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PRODUCT NAME |
Calpain small subunit 1 Monoclonal Antibody |
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CONJUGATION |
Unconjugated |
|
HOST |
Rabbit |
| SOURCE |
Recombinant protein within N-terminal human Calpain small subunit 1. |
| IMMUNOGEN RANGE |
|
| CLONALITY |
Monoclonal |
| ISOTYPE |
IgG |
| CONCENTRATION |
1ug/ul |
| PURIFICATION |
Purified by Protein A. |
| STORAGE BUFFER |
Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. |
| STORAGE CONDITION |
Store at -20°C for 12 months.. |
TARGET |
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| GENE ID |
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| SUBCELLULAR LOCATION |
Cytoplasm, Membrane |
| SYNONYMS |
30K antibody, Calcium activated neutral proteinase antibody, Calcium activated neutral proteinase small subunit antibody, Calcium dependent protease small subunit 1 antibody, Calcium dependent protease small subunit antibody, Calcium-activated neutral proteinase small subunit antibody, Calcium-dependent protease small subunit 1 antibody, Calcium-dependent protease small subunit antibody, Calpain 4 antibody, Calpain 4 small subunit (30K) antibody, Calpain 4 small subunit antibody, Calpain regulatory subunit antibody, Calpain S1 antibody, Calpain small polypeptide antibody, Calpain small subunit 1 antibody, Calpain4 antibody, CalpainS1 antibody, CANP antibody, CANP small subunit antibody, CANPS antibody, CAPN 4 antibody, CAPN S1 antibody, CAPN4 antibody, CAPNS 1 antibody, CAPNS antibody, Capns1 antibody, CDPS antibody, CPNS1_HUMAN antibody, CSS 1 antibody, CSS1 antibody |
| BACKGROUND |
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| APPLICATION DILUTION |
WB(1:300-1000), FCM(1:20-100), IHC-P(1:200-400), IHC() |