Mouse Anti-Chicken IgY-Alexa Fluor® 647



Datasheet      Tech Support









Antibody Testing Data:

FLISA plate was coated with purified chicken IgY and IgM. Immunoglobulins were detected with serially diluted Mouse Anti-Chicken IgY-AF647 (SB Cat. No. 8320-31).








Product Details






Product Specifications

Name:

Mouse Anti-Chicken IgY-Alexa Fluor® 647

Clone:

G-1

Isotype:

Mouse (BALB/c) IgG1κ (Isotype Control:Mouse IgG1-Alexa Fluor® 647)

Specificity:

Chicken/Turkey IgY

Format/Conjugate:

AF647 (Alexa Fluor® 647)

Buffer Formulation:

Phosphate buffered saline containing 0.1% sodium azide

Concentration:

0.5 mg/mL

Volume:

0.2 mL

Storage & Handling:

2-8°C; Avoid exposure to light blue Please refer to product specific SDS.

RRID:

AB_2796499

Recommended Dilutions:

Please refer to product specific Technical Guide.

Applications:

Applications for relevant formats of this clone include -
ELISA – Quality tested 6-9
FLISA – Quality tested
Flow Cytometry – Reported in literature 10-13
Immunohistochemistry-Frozen Sections – Reported in literature 2-4
Immunocytochemistry – Reported in literature 5
Immunoprecipitation – Reported in literature 1

More information:

Immunogen: Affinity purified chicken Ig or isolated lymphocytes
Alternalte Name(s): Chicken IgG, Turkey IgG

*For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
















References






1. Chen CH, Lehmeyer JE, Cooper MD. Evidence for an IgD homologue on chicken lymphocytes. J Immunol. 1982;129:2580-5. (Immunogen, IP)

2. Javed MA, Frasca S Jr, Rood D, Cecchini K, Gladd M, Geary SJ, et al. Correlates of immune protection in chickens vaccinated with Mycoplasma gallisepticum strain GT5 following challenge with pathogenic M. gallisepticum strain Rlow. Infect Immun. 2005;73:5410-9. (IHC-FS)

3. Hansell C, Zhu XW, Brooks H, Sheppard M, Withanage S, Maskell D, et al. Unique features and distribution of the chicken CD83+ cell. J Immunol. 2007;179:5117-25. (IHC-FS)

4. Bader SR, Kothlow S, Trapp S, Schwarz SC, Philipp H, Weigend S, et al. Acute paretic syndrome in juvenile White Leghorn chickens resembles late stages of acute inflammatory demyelinating polyneuropathies in humans. J Neuroinflammation. 2010;7:7. (IHC-FS)

5. Singh S, Briles WE, Lupiani B, Collisson EW. Avian influenza viral nucleocapsid and hemagglutinin proteins induce chicken CD8+ memory T lymphocytes. Virology. 2010;399:231-8. (ICC)

6. Bailey JS, Rolón A, Hofacre CL, Holt PS, Wilson JL, Cosby DE, et al. Intestinal humoral immune response and resistance to Salmonella challenge of progeny from breeders vaccinated with killed antigen. Intl J Poult Sci. 2007;6:417-23. (ELISA)

7. Fasina YO, Holt PS, Moran ET, Moore RW, Conner DE, McKee SR. Intestinal cytokine response of commercial source broiler chicks to Salmonella typhimurium infection. Poult Sci. 2008;87:1335-46. (ELISA)

8. Lardinois A, van den Berg T, Lambrecht B, Steensels M. A model for the transfer of passive immunity against Newcastle disease and avian influenza in specific pathogen free chickens. Avian Pathol. 2014;43:118-24. (ELISA)

9. Orr-Burks N, Gulley SL, Gallardo RA, Toro H, van Ginkel FW. Immunoglobulin A as an early humoral responder after mucosal avian coronavirus vaccination. Avian Dis. 2014;58:279-86. (ELISA)

10. Del Cacho E, Gallego M, López-Bernard F, Sánchez-Acedo C, Lillehoj HS. Isolation of chicken follicular dendritic cells. J Immunol Methods. 2008;334:59-69. (FC)

11. Del Cacho E, Gallego M, Lillehoj HS, López-Bernard F, Sánchez-Acedo C. Avian follicular and interdigitating dendritic cells: isolation and morphologic, phenotypic, and functional analyses. Vet Immunol Immunopathol. 2009;129:66-75. (FC)

12. Janardhana V, Broadway MM, Bruce MP, Lowenthal JW, Geier MS, Hughes RJ, et al. Prebiotics modulate immune responses in the gut-associated lymphoid tissue of chickens. J Nutr. 2009;139:1404-9. (FC)

13. Petkov DI, Linnemann EG, Kapczynski DR, Sellers HS. Identification and characterization of two distinct bursal B-cell subpopulations following infectious bursal disease virus infection of White Leghorn chickens. Avian Dis. 2009;53:347-55. (FC)
Alexa Fluor® is a registered trademark of Life Technologies Corporation










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