Mouse Anti-Chicken IgM-UNLB



Datasheet      Tech Support









Antibody Testing Data:

FLISA plate was coated with purified chicken IgM and IgY. Immunoglobulins were detected with serially diluted Mouse Anti-Chicken IgM-PE (SB Cat. No. 8310-09).








Product Details






Product Specifications

Name:

Mouse Anti-Chicken IgM-UNLB

Clone:

M-1

Isotype:

Mouse (BALB/c) IgG2bκ ( Isotype Control: Mouse IgG2b-UNLB)

Specificity:

Chicken/Turkey IgM

Format/Conjugate:

UNLB (Unconjugated)

Buffer Formulation:

Borate buffered saline, pH 8.2

Concentration:

0.5 mg/mL

Volume:

1.0 mL

Storage & Handling:

2-8°C blue Please refer to product specific SDS.

RRID:

AB_2796488

Recommended Dilutions:

Please refer to product specific Technical Guide.

Applications:

Applications for relevant formats of this clone include -
Flow Cytometry – Quality tested 2,3,7-20
ELISA – Quality tested
FLISA – Quality tested
Immunohistochemistry-Frozen Sections – Reported in literature 2-4
Immunocytochemistry – Reported in literature 1
Immunoprecipitation – Reported in literature 1,2,5
Stimulation – Reported in literature 6

More information:

Immunogen: Affinity purified chicken Ig or isolated lymphocytes
Gene ID: 107050812 (Chicken)
Gene Symbol : LOC107050812 (Chicken)
Uniprot ID: P01875 (Chicken)
Uniprot Name: IGHM_CHICK

*For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
















References






1. Chen CH, Lehmeyer JE, Cooper MD. Evidence for an IgD homologue on chicken lymphocytes. J Immunol. 1982;129:2580-5. (Immunogen, IP, ICC)

2. Ekino S, Riwar B, Kroese FG, Schwander EH, Koch G, Nieuwenhuis P. Role of environmental antigen in the development of IgG+ cells in the bursa of fabricius. J Immunol. 1995;155:4551-8. (FC, IP, IHC-FS)

3. Schusser B, Collarini EJ, Yi H, Izquierdo SM, Fesler J, Pedersen D, et al. Immunoglobulin knockout chickens via efficient homologous recombination in primordial germ cells. Proc Natl Acad Sci USA. 2013;110:20170-5. (IHC-FS, FC)

4. Bader SR, Kothlow S, Trapp S, Schwarz SC, Philipp H, Weigend S, et al. Acute paretic syndrome in juvenile White Leghorn chickens resembles late stages of acute inflammatory demyelinating polyneuropathies in humans. J Neuroinflammation. 2010;7:7. (IHC-FS, FC)

5. Alinikula J, Nera K, Junttila S, Lassila O. Alternate pathways for Bcl6-mediated regulation of B cell to plasma cell differentiation. Eur J Immunol. 2011;41:2404-13. (IP)

6. Viertlboeck BC, Schweinsberg S, Hanczaruk MA, Schmitt R, Du Pasquier L, Herberg FW, et al. The chicken leukocyte receptor complex encodes a primordial, activating, high-affinity IgY Fc receptor. Proc Natl Acad Sci USA. 2007;104:11718-23. (Stim)

7. Wang YW, Cherian G, Sunwoo HH, Sim JS. Dietary polyunsaturated fatty acids significantly affect laying hen lymphocyte proliferation and immunoglobulin G concentration in serum and egg yolk. Can J Anim Sci. 2000;80:597-604. (FC)

8. Selvaraj RK, Cherian G. Dietary n-3 fatty acids reduce the delayed hypersensitivity reaction and antibody production more than n-6 fatty acids in broiler birds. Eur J Lipid Sci Technol. 2004;106:3-10. (FC)

9. Selvaraj RK, Cherian G. Changes in delayed type hypersensitivity, egg antibody content and immune cell fatty acid composition of layer birds fed conjugated linoleic acid, n-6 or n-3 fatty acids. Can J Anim Sci. 2004;84:221-8. (FC)

10. Chowdhury SR, Smith TK, Boermans HJ, Woodward B. Effects of feed-borne Fusarium mycotoxins on hematology and immunology of laying hens. Poult Sci. 2005;84:1841-50. (FC)

11. Nera K, Alinikula J, Terho P, Narvi E, Törnquist K, Kurosaki T, et al. Ikaros has a crucial role in regulation of B cell receptor signaling. Eur J Immunol. 2006;36:516-25. (FC)

12. Yang SY, Fugmann SD, Schatz DG. Control of gene conversion and somatic hypermutation by immunoglobulin promoter and enhancer sequences. J Exp Med. 2006;203:2919-28. (FC)

13. Cook AJ, Raftery JM, Lau KK, Jessup A, Harris RS, Takeda S, et al. DNA-dependent protein kinase inhibits AID-induced antibody gene conversion. PLoS Biol. 2007;5(4):e80. (FC)

14. Yang SY, Fugmann SD, Gramlich HS, Schatz DG. Activation-induced cytidine deaminase-mediated sequence diversification is transiently targeted to newly integrated DNA substrates. J Biol Chem. 2007;282:25308-13. (FC)

15. Janardhana V, Broadway MM, Bruce MP, Lowenthal JW, Geier MS, Hughes RJ, et al. Prebiotics modulate immune responses in the gut-associated lymphoid tissue of chickens. J Nutr. 2009;139:1404-9. (FC)

16. Petkov DI, Linnemann EG, Kapczynski DR, Sellers HS. Identification and characterization of two distinct bursal B-cell subpopulations following infectious bursal disease virus infection of White Leghorn chickens. Avian Dis. 2009;53:347-55. (FC)

17. Luo J, Zhang H, Teng M, Fan J, You L, Xiao Z, et al. Surface IgM on DT40 cells may be a component of the putative receptor complex responsible for the binding of infectious bursal disease virus. Avian Pathol. 2010;39:359-65. (FC)

18. Jankowski J, Zduńczyk Z, Sartowska K, Tykałowski B, Stenzel T, Wróblewska M, et al. Metabolic and immune response of young turkeys originating from parent flocks fed diets with inorganic or organic selenium. Pol J Vet Sci. 2011;14:353-8. (FC, Turkey Reactivity)

19. Levkut M, Revajová V, Lauková A, Ševčíková Z, Spišáková V, Faixová Z, et al. Leukocytic responses and intestinal mucin dynamics of broilers protected with Enterococcus faecium EF55 and challenged with Salmonella Enteritidis. Res Vet Sci. 2012;93:195-201. (FC)

20. Revajová V, Slaminková Z, Grešáková L, Levkut M. Duodenal morphology and immune responses of broiler chickens fed low doses of deoxynivalenol. Acta Vet. Brno. 2013;82:337-42. (FC)

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