Mouse Anti-Chicken IgA-BIOT



Datasheet      Tech Support









Antibody Testing Data:

Frozen chicken intestine tissue section was stained with Mouse Anti-Chicken IgA-BIOT (SB Cat. No. 8330-08) followed by Streptavidin-CY3 (SB Cat. No. 7100-12) and DAPI.








Product Details






Product Specifications

Name:

Mouse Anti-Chicken IgA-BIOT

Clone:

A-1

Isotype:

Mouse (BALB/c) IgG2bκ (Isotype Control:Mouse IgG2b-BIOT)

Specificity:

Chicken IgA

Format/Conjugate:

BIOT (Biotin)

Buffer Formulation:

Phosphate buffered saline containing 0.1% sodium azide

Concentration:

0.5 mg/mL

Volume:

1.0 mL

Storage & Handling:

2-8°C blue Please refer to product specific SDS.

RRID:

AB_2796502

Recommended Dilutions:

Please refer to product specific Technical Guide.

Applications:

Applications for relevant formats of this clone include -
Immunocytochemistry – Quality tested
Flow Cytometry – Reported in literature 25,26
Immunohistochemistry-Frozen Sections – Reported in literature 3,4
Immunohistochemistry-Paraffin Sections – Reported in literature 5-16,25
Immunoprecipitation – Reported in literature 1,2
ELISA – Reported in literature 18-25

More information:

Immunogen: Affinity purified chicken Ig or isolated lymphocytes

*For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
















References






1. Chen CH, Lehmeyer JE, Cooper MD. Evidence for an IgD homologue on chicken lymphocytes. J Immunol. 1982;129:2580-5. (Immunogen, IP)
2. van Ginkel FW, Tang DC, Gulley SL, Toro H. Induction of mucosal immunity in the avian Harderian gland with a replication-deficient Ad5 vector expressing avian influenza H5 hemagglutinin. Dev Comp Immunol. 2009;33:28-34. (IP)
3. Fahey KJ, York JJ. The role of mucosal antibody in immunity to infectious laryngotracheitis virus in chickens. J Gen Virol. 1990;71:2401-5. (IHC-FS)
4. Javed MA, Frasca S Jr, Rood D, Cecchini K, Gladd M, Geary SJ, et al. Correlates of immune protection in chickens vaccinated with Mycoplasma gallisepticum strain GT5 following challenge with pathogenic M. gallisepticum strain Rlow. Infect Immun. 2005;73:5410-9. (IHC-FS)
5. Yurong Y, Yibao J, Ruiping S, Qingqiang Y, Kaisong P, Huihui B, et al. Effects of chicken intestinal antimicrobial peptides on humoral immunity of chickens and antibody titres after vaccination with infectious bursal disease virus vaccine in chicken. Arch Anim Nutr. 2006;60:427-35. (IHC-PS)
6. Gao J, Zhang HJ, Yu SH, Wu SG, Yoon I, Quigley J, et al. Effects of yeast culture in broiler diets on performance and immunomodulatory functions. Poult Sci. 2008;87:1377-84. (IHC-PS)
7. Liu T, She R, Wang K, Bao H, Zhang Y, Luo D, et al. Effects of rabbit sacculus rotundus antimicrobial peptides on the intestinal mucosal immunity in chickens. Poult Sci. 2008;87:250-4. (IHC-PS)
8. Wang D, Ma W, She R, Sun Q, Liu Y, Hu Y, et al. Effects of swine gut antimicrobial peptides on the intestinal mucosal immunity in specific-pathogen-free chickens. Poult Sci. 2009;88:967-74. (IHC-PS)
9. Wang D, Zhou X, She R, Xiong J, Sun Q, Peng K, et al. Impaired intestinal mucosal immunity in specific-pathogen-free chickens after infection with very virulent infectious bursal disease virus. Poult Sci. 2009;88:1623-8. (IHC-PS)
10. Jiang YB, Yin QQ, Yang YR. Effect of soybean peptides on growth performance, intestinal structure and mucosal immunity of broilers. J Anim Physiol Anim Nutr (Berl). 2009;93:754-60. (IHC-PS)
11. Liu P, Shi W, Zhao Y, Zhong X . Effects of Chinese herbal formula Maxing Shigan powder on IgA secreting cells in chicken bronchus. J Med Plants Res. 2011;5:4304-9. (IHC-PS)
12. Yan Z, Du Y, Zhao Q, Fan R, Guo W, Ma R, et al. Mucosal immune responses against live Newcastle disease vaccine in immunosuppressed chickens. Pak Vet J. 2011;31:280-6. (IHC-PS)
13. Cheng J, Li Q, Shi W, Zhong X. Effects of Huangqi Maxingshigan decoction on infectious laryngotracheitis in chickens. Ital J Anim Sci. 2011;10:124-30. (IHC-PS)
14. Deng W, Dong XF, Tong JM, Zhang Q. The probiotic Bacillus licheniformis ameliorates heat stress-induced impairment of egg production, gut morphology, and intestinal mucosal immunity in laying hens. Poult Sci. 2012;91:575-82. (IHC-PS)
15. Zhang D, Shi W, Zhao Y, Zhong X. Adjuvant effects of Sijunzi decoction in chickens orally vaccinated with attenuated Newcastle-disease vaccine. Afr J Tradit Complement Altern Med. 2012;9:120-30. (IHC-PS)
16. Liu J, Cui H, Peng X, Fang J, Zuo Z, Deng J, et al. Decreased IgA+ B cells population and IgA, IgG, IgM contents of the cecal tonsil induced by dietary high fluorine in broilers. Int J Environ Res Public Health. 2013;10:1775-85. (IHC-PS)
17. Muir WI, Husband AJ, Bryden WL. Dietary supplementation with vitamin E modulates avian intestinal immunity. Br J Nutr. 2002;87:579-85. (ELISA)
18. Bailey JS, Rolón A, Hofacre CL, Holt PS, Wilson JL, Cosby DE, et al. Intestinal humoral immune response and resistance to Salmonella challenge of progeny from breeders vaccinated with killed antigen. Intl J Poult Sci. 2007;6:417-23. (ELISA)
19. Fasina YO, Holt PS, Moran ET, Moore RW, Conner DE, McKee SR. Intestinal cytokine response of commercial source broiler chicks to Salmonella typhimurium infection. Poult Sci. 2008;87:1335-46. (ELISA)
20. Vaughn LE, Holt PS, Moore RW, Gast RK, Anderson KE. Crop immune response post-Salmonella enteritidis challenge in eight commercial egg-layer strains and specific-pathogen-free White Leghorn chickens. Avian Dis. 2008;52:79-87. (ELISA)
21. Fassbinder-Orth CA, Hofmeister EK, Weeks-Levy C, Karasov WH. Oral and parenteral immunization of chickens (Gallus gallus) against West Nile virus with recombinant envelope protein. Avian Dis. 2009;53:502-9. (ELISA)
22. Ishola OO, Ogundipe GA. Salmonella Enterica serovar Enteritidis experimental infection in chickens: effects of the infectious dose on crop immunoglobulin A antibody response. Isr J Vet Med. 2009;64:6-11. (ELISA)
23. Lardinois A, van den Berg T, Lambrecht B, Steensels M. A model for the transfer of passive immunity against Newcastle disease and avian influenza in specific pathogen free chickens. Avian Pathol. 2014;43:118-24. (ELISA)
24. Orr-Burks N, Gulley SL, Gallardo RA, Toro H, van Ginkel FW. Immunoglobulin A as an early humoral responder after mucosal avian coronavirus vaccination. Avian Dis. 2014;58:279-86. (ELISA)
25. Janardhana V, Broadway MM, Bruce MP, Lowenthal JW, Geier MS, Hughes RJ, et al. Prebiotics modulate immune responses in the gut-associated lymphoid tissue of chickens. J Nutr. 2009;139:1404-9. (ELISA, IHC-PS, FC)
26. Petkov DI, Linnemann EG, Kapczynski DR, Sellers HS. Identification and characterization of two distinct bursal B-cell subpopulations following infectious bursal disease virus infection of White Leghorn chickens. Avian Dis. 2009;53:347-55. (FC)










We're here to help


Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.


Contact Tech Support







High Quality Guaranteed Product


Our products such as Elisa, Antibodies, Proteins, Peptides are covered by Biolinkk quality warranty and will work as described in datasheet, a free replacement or money back is guaranteed if does not perform according to datasheet.


Learn More