Anti-Mouse Ly-6G/Ly-6C In Vivo Antibody – Ultra Low Endotoxin (RB6-8C5)
- Sizes: 5mg, 25mg, 50mg, 100mg,
- Category: ichorbio, Ultra Low Endotoxin, anti-mouse
- Target: Ly-6G/Ly-6C
- Clone: RB6-8C5
- Host: Rat
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Datasheet
Tech Support
PRODUCT DETAILS
SPECIFICATIONS |
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PRODUCT NAME |
Anti-Mouse Ly-6G/Ly-6C In Vivo Antibody - Ultra Low Endotoxin (RB6-8C5) |
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SPECIES REACTIVITY |
Mouse |
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HOST |
Rat |
| SPECIFICITY |
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| PURIFICATION METHOD |
This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. |
| ANTIGEN DISTRIBUTION |
The Gr-1 antigen is present at various levels correlated with granulocyte differentiation and maturation. The Gr-1 antigen is expressed on other myeloid populations, but not on lymphoid or erythroid cells. |
| IMMUNOGEN |
Raised against granulocytes of mouse origin |
| CONCENTRATION |
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| FORMULATION |
No carrier proteins or preservatives added in 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl. |
| PURITY |
>98% by SDS-PAGE and HPLC |
| ENDOTOXIN |
≤ 0.75 EU/mg as determined by the LAL method |
| AGGRIGATION |
Aggregation level ≤ 1% |
| STORAGE CONDITION |
anti-Ly-6G/Ly-6C In Vivo Antibody - Ultra Low Endotoxin (RB6-8C5) is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at – 20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. |
TARGET |
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| TARGET |
Ly-6G/Ly-6C |
| CLONE |
RB6-8C5 |
| SYNONYMS |
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| BACKGROUND |
Gr-1 is a 21-25 kD protein also known as Ly-6G/Ly-6C. This myeloid differentiation antigen is a glycosylphosphatidylinositol (GPI)-linked protein expressed on granulocytes and macrophages. Clone RB6-8C5 antibody has been shown to inhibit the binding of the clone 1A8 antibody. Clone 1A8 monoclonal antibody reacts specifically with mouse Ly6G with no reported cross-reactivity with Ly6C. |
| UNIPROT |
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| PATHOGEN TEST |
We use the IMPACT test generated by IDEXX Laboratories to guarantee our Ultra Low Endotoxin antibodies are pathogen free. Our rat antibodies are tested for: |
| APPLICATIONS |
Immunoprecipitation, Western Blot, Flow Cytometry, Functional Assays, IHC (Frozen), IHC (Paraffin), Immunocytochemistry, Immunofluorescence, In Situ Hybridization |
| APPLICATION NOTES |
Each investigator should determine their own optimal working dilution for specific applications. |
| USAGE |
Products are for research use only. Not for use in diagnostic or therapeutic procedures. |