Anti-Mouse Ly-6G/Ly-6C In Vivo Antibody – Low Endotoxin (RB6-8C5)
- Sizes: 5mg, 25mg, 50mg, 100mg,
- Category: ichorbio, Low Endotoxin, anti-mouse
- Target: Ly-6G/Ly-6C
- Clone: RB6-8C5
- Host: Rat
-
Datasheet Tech Support
PRODUCT DETAILS
SPECIFICATIONS |
|
---|---|
PRODUCT NAME |
Anti-Mouse Ly-6G/Ly-6C In Vivo Antibody - Low Endotoxin (RB6-8C5) |
SPECIES REACTIVITY |
Mouse |
HOST |
Rat |
SPECIFICITY |
|
PURIFICATION METHOD |
This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. |
ANTIGEN DISTRIBUTION |
The Gr-1 antigen is present at various levels correlated with granulocyte differentiation and maturation. The Gr-1 antigen is expressed on other myeloid populations, but not on lymphoid or erythroid cells. |
IMMUNOGEN |
Raised against granulocytes of mouse origin |
CONCENTRATION |
|
FORMULATION |
No carrier proteins or preservatives added in 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl. |
PURITY |
>95% by SDS-PAGE and HPLC |
ENDOTOXIN |
≤ 1.0 EU/mg as determined by the LAL method |
AGGRIGATION |
Aggregation level ≤ 5% |
STORAGE CONDITION |
anti-Ly-6G/Ly-6C In Vivo Antibody - Low Endotoxin (RB6-8C5) is stable for at least four (4) week when stored at 2-8°C. For long-term storage, aliquot in working volumes without diluting and store at – 20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. |
TARGET |
|
---|---|
TARGET |
Ly-6G/Ly-6C |
CLONE |
RB6-8C5 |
SYNONYMS |
|
BACKGROUND |
Gr-1 is a 21-25 kD protein also known as Ly-6G/Ly-6C. This myeloid differentiation antigen is a glycosylphosphatidylinositol (GPI)-linked protein expressed on granulocytes and macrophages. Clone RB6-8C5 antibody has been shown to inhibit the binding of the clone 1A8 antibody. Clone 1A8 monoclonal antibody reacts specifically with mouse Ly6G with no reported cross-reactivity with Ly6C. |
UNIPROT |
|
PATHOGEN TEST |
|
APPLICATIONS |
Immunoprecipitation, Western Blot, Flow Cytometry, Functional Assays, IHC (Frozen), IHC (Paraffin), Immunocytochemistry, Immunofluorescence, In Situ Hybridization |
APPLICATION NOTES |
Each investigator should determine their own optimal working dilution for specific applications. |
USAGE |
Products are for research use only. Not for use in diagnostic or therapeutic procedures. |