PRODUCT NAME

Anti-Mouse, Human CD45R In Vivo Antibody - Ultra Low Endotoxin (RA3-6B2)

SPECIES REACTIVITY

Mouse, Human

HOST

Rat

SPECIFICITY

Anti-CD45R In Vivo Antibody - Ultra Low Endotoxin (RA3-6B2) recognizes the B-cell determinant (Mr 220 kD) of the CD45 molecule.

PURIFICATION METHOD

ANTIGEN DISTRIBUTION

The CD45R antigen is present on mouse B-cells, B-cell precursors, T cell subset, NK cell subsets .

IMMUNOGEN

Abelson murine leukemia virus-induced pre-B tumor cells

CONCENTRATION

FORMULATION

No carrier proteins or preservatives added in 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl.

PURITY

>98% by SDS-PAGE and HPLC

ENDOTOXIN

≤ 0.75 EU/mg as determined by the LAL method

AGGRIGATION

Aggregation level ≤ 1%

STORAGE CONDITION

anti-CD45R In Vivo Antibody - Ultra Low Endotoxin (RA3-6B2) is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.

TARGET

CD45R

CLONE

RA3-6B2

SYNONYMS

BACKGROUND

CD45R, also known as B220 is a 220 kDa transmembrane protein tyrosine phosphatase expressed on B cells and some subsets of T and NK cells. This antibody is commonly used as a B cell marker as CD45R does play a critical role in TCR and BCR signaling.

UNIPROT

P08575

PATHOGEN TEST

We use the IMPACT test generated by IDEXX Laboratories to guarantee our Ultra Low Endotoxin antibodies are pathogen free. Our rat antibodies are tested for:
Mycoplasma spp
Mycoplasma pulmonis
Pneumonia virus of mice
Kilham's rat virus
Toolan's H1 virus
Rat parvovirus
Lymphocytic choriomeningitis virus
Rat cytomegalovirus
Sendai virus
Rat coronavirus
Sialodacryoadenitis virus
Rat minute virus
Seoul virus
Mouse adenovirus
Reovirus 3
Rat theilovirus

APPLICATIONS

Western Blot, Functional Assays, Flow Cytometry, IHC (Frozen), IHC (Paraffin), Immunoprecipitation, Immunofluorescence, ELISA

APPLICATION NOTES

Functional Assay: In vivo treatment with Rat Anti-Mouse CD45R (Clone RA3-6B2) reduces the number of T and B cells and modifies their functional activity.
Each investigator should determine their own optimal working dilution for specific applications.

USAGE

Products are for research use only. Not for use in diagnostic or therapeutic procedures.