Anti-Mouse IL-1R In Vivo Antibody – Low Endotoxin (JAMA-147)
- Sizes: 5mg, 25mg, 50mg, 100mg,
- Category: ichorbio, Low Endotoxin, anti-mouse
- Target: IL1R1
- Clone: JAMA-147
- Host: Armenian Hamster
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Datasheet
Tech Support
PRODUCT DETAILS
SPECIFICATIONS |
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PRODUCT NAME |
Anti-Mouse IL-1R In Vivo Antibody - Low Endotoxin (JAMA-147) |
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SPECIES REACTIVITY |
Mouse |
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HOST |
Armenian Hamster |
| SPECIFICITY |
Anti-IL-1R In Vivo Antibody - Low Endotoxin (JAMA-147) recognizes Mouse IL-1 R1 |
| PURIFICATION METHOD |
This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. |
| ANTIGEN DISTRIBUTION |
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| IMMUNOGEN |
Extracellular domain of mouse IL-1 R type 1 |
| CONCENTRATION |
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| FORMULATION |
0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added. |
| PURITY |
>95% by SDS-PAGE and HPLC |
| ENDOTOXIN |
≤ 1.0 EU/mg as determined by the LAL method |
| AGGRIGATION |
Aggregation level ≤ 5% |
| STORAGE CONDITION |
anti-IL-1R In Vivo Antibody - Low Endotoxin (JAMA-147) is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles |
TARGET |
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| TARGET |
IL1R1 |
| CLONE |
JAMA-147 |
| SYNONYMS |
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| BACKGROUND |
IL-1 binds two receptors, IL-1RI and IL-1RII. The ligands IL-1alpha and IL-1beta exert all of their various biological actions by binding IL-1RI. IL-1RII has a shortened cytoplasmic domain that is unable to transduce signals and therefore acts as a decoy receptor and competitively antagonizes the binding of IL-1alpha and IL-1beta, thereby reducing their biological effects. Binding of the ligands to IL-1RI is enhanced by an accessory protein, IL-1R-AcP. IL-1RI is expressed on T cells, thymocytes, dendritic cells, fibroblasts, vascular endothelial cells, epithelial cells and neural cells. It is an 80 kD transmembrane glycoprotein. |
| UNIPROT |
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| PATHOGEN TEST |
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| APPLICATIONS |
Immunoprecipitation, Western Blot, Blocking, Flow Cytometry |
| APPLICATION NOTES |
Each investigator should determine their own optimal working dilution for specific applications. |
| USAGE |
Products are for research use only. Not for use in diagnostic or therapeutic procedures. |