Anti-Mouse CD44 In Vivo Antibody – Low Endotoxin (IM7)
- Sizes: 5mg, 25mg, 50mg, 100mg,
- Category: ichorbio, Low Endotoxin, anti-mouse
- Target: CD44
- Clone: IM7
- Host: Rat
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Datasheet
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PRODUCT DETAILS
SPECIFICATIONS |
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PRODUCT NAME |
Anti-Mouse CD44 In Vivo Antibody - Low Endotoxin (IM7) |
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SPECIES REACTIVITY |
Mouse |
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HOST |
Rat |
| SPECIFICITY |
Anti-CD44 In Vivo Antibody - Low Endotoxin (IM7) recognizes an epitope common to alloantigens & all isoforms of CD44, that is located between amino acids 145 and 186 |
| PURIFICATION METHOD |
This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. |
| ANTIGEN DISTRIBUTION |
The CD44 antigen is present on B-cells, monocytes, macrophages and certain subsets of thymocytes and peripheral T-cells. Mice with the Ly-24.1 allotype have high densities of CD44+ T-cells, while Ly-24.2 strains are low expressors. The antibody cross reacts with: Ms+, Hu+, Bov+, Mky+, Bab+, Dog+, Cat, Porcine |
| IMMUNOGEN |
Dexamethasone-induced myeloid leukemia M1 cells |
| CONCENTRATION |
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| FORMULATION |
0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added. |
| PURITY |
>95% by SDS-PAGE and HPLC |
| ENDOTOXIN |
≤ 1.0 EU/mg as determined by the LAL method |
| AGGRIGATION |
Aggregation level ≤ 5% |
| STORAGE CONDITION |
anti-CD44 In Vivo Antibody - Low Endotoxin (IM7) is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles. |
TARGET |
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| TARGET |
CD44 |
| CLONE |
IM7 |
| SYNONYMS |
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| BACKGROUND |
CD44 is a 80-95 kD glycoprotein also known as Hermes, Pgp1, H-CAM, or HUTCH. It is expressed on all leukocytes, endothelial cells, hepatocytes, and mesenchymal cells. As B and T cells become activated or progress to the memory stage, CD44 expression increases from low or mid levels to high levels. Clone IM7 antibody has been shown to neutralize CD44 in vivo |
| UNIPROT |
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| PATHOGEN TEST |
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| APPLICATIONS |
Immunoprecipitation, Western Blot, Flow Cytometry, CyTOF, IHC (Frozen), ELISA, Functional Assays |
| APPLICATION NOTES |
Each investigator should determine their own optimal working dilution for specific applications. |
| USAGE |
Products are for research use only. Not for use in diagnostic or therapeutic procedures. |