Catalog#: 31-1030-MK
Applications: Sandwich ELISA, or other Sandwich Assays
Price Not Available
Request bulk or custom quote
Product Details
Product Specifications |
|
|---|---|
|
Product Name |
Human IgA ELISA Matched Antibody Pair |
| Product Description: |
The Human IgA Matched Antibody Pairs is specific to Human IgA and has been demonstrated through ELISA to not recognize (cross) IgG from Non-Human Primate species (Rhesus and Cynomologus macaques), nor does the matched pair recognize mouse, rat or goat immunoglobulin. The kit can be used for the detection of therapeutic antibodies from samples obtained from experimental animals to provide accurate pharmacokinetic (PK) data. |
|
Catalog No. |
31-1030-MK |
|
Clone Name |
RM128 Anti-Human IgG4 Rabbit Monoclonal AntibodyRM129 Biotin Anti-Human Ig Light Chain Rabbit Monoclonal Antibody |
| Product Datasheet |
|
| Specificity |
The antibody pair detects only human IgA. It does not react to monkey (Cyno or Rhesus) IgG, mouse IgG, rat IgG, or goat IgG. There is no cross reactivity to any other immunoglobulins or IgG subclasses. |
|
Immunogen |
Human IgA |
|
Application |
Sandwich ELISA, or other Sandwich Assays |
|
Species Reactivity |
Human |
| Conjugate |
RM129: Biotin |
|
Intended Use |
Research Use Only |
Properties |
|
|---|---|
|
Host |
Rabbit |
|
Storage Instruction |
Store at -20.0°C |
| Stability |
Stable for 1 Year at -20.0°C from date of receipt |
Storage Buffer |
50% Glycerol/PBS with 1% BSA and 0.09% sodium azide |
Concentration |
1) Capture Antibody: Anti-Human IgG4 Rabbit Monoclonal antibody, clone RM120, 100 ug at 1.0 mg/mL in 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide;2) Detection Antibody: Biotin Anti-Human Ig Light Chain Rabbit Monoclonal Antibody, Clone RM129, 25 ug at 1.0 mg/mL in 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide;3) 10X Sample Diluent: Added to the tested sample to reduce non-specific background and noise |
Volume/Size |
|
| Purity |
Protein A affinity purified from an animal origin-free culture supernatant |
|
Clone Type |
Recombinant Monoclonal |
|
Isotype |
Rabbit IgG |
|
Uniport |
|
|
Gene ID |
3493/3494 |
| Usage |
|
|
Keywords |
- |
*For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Product Images
Sandwich ELISA using RM128 as the capture antibody (100ng/well), and Biotinylated anti-human light chains (k+l) antibody RM129 (0.2ug/mL) as the detection antibody, followed by an alkaline phosphatase conjugated streptavidin.
Sandwich ELISA, using RevMAb human IgA matched antibody pair (capture: RM128; detection: Biotin-RM129), shows species reactivity to human only, and shows no cross-reactivity to monkey (Cyno or Rhesus), mouse IgA, rat IgA, or goat IgA.
Sandwich ELISA, using RevMAb human IgA matched antibody pair, shows no cross- reactivity with Human IgG, IgE, IgM, or IgD. The plate was coated with human IgA capture antibody (50ng/well). Different immunoglobulin samples (0.2 ug/mL) were added and biotinylated RM129 (0.2ug/mL) was used as detection antibody, followed by an alkaline phosphatase conjugated streptavidin