Goat Anti-Equine IgG(H+L)-AP



Datasheet      Tech Support









Antibody Testing Data:

ELISA plate was coated with purified horse IgG. Immunoglobulin was detected with Goat Anti-Equine IgG(H+L)-AP (SB Cat. No. 6040-04).








Product Details






Product Specifications

Name:

Goat Anti-Equine IgG(H+L)-AP

Clone:

-

Isotype:

Goat IgG

Specificity:

Reacts with the heavy and light chains of equine IgG

Format/Conjugate:

AP (Alkaline Phosphatase)

Buffer Formulation:

50 mM Tris/1 mM MgCl2/50% Glycerol containing 0.1% sodium azide, pH 8.0

Concentration:

-

Volume:

1.0 mL

Storage & Handling:

2-8°C blue Please refer to product specific SDS.

RRID:

AB_2796116

Recommended Dilutions:

Please refer to product specific Technical Guide.

Applications:

Quality tested applications for relevant formats include -
ELISA 1-3
FLISA
Other referenced applications for relevant formats include -
Flow Cytometry 4,5
Immunocytochemistry 6
Western Blot 7-10

More information:

Source: Pooled antisera from goats hyperimmunized with equine IgG
Cross Adsorption: None; may react with immunoglobulins from other species and the light chains of other equine immunoglobulins
Purification: Affinity chromatography on donkey IgG covalently linked to agarose

*For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
















References






1. Hobo S, Niwa H, Anzai T, Jones JH. Changes in serum antibody levels after vaccination for strangles and after intranasal challenge with Streptococcus equi subsp. equi in horses. J Equine Sci. 2010;21:33-7. (ELISA)

2. De Keyser K, Oosterlinck M, Raes E, Ducatelle R, Janssens S, Buys N. Early detection of chronic progressive lymphoedema susceptibility in Belgian draught horse stallions by means of ELISA. Commun Agric Appl Biol Sci. 2012;77:183-7. (ELISA)

3. De Keyser K, Berth M, Christensen N, Willaert S, Janssens S, Ducatelle R, et al. Assessment of plasma anti-elastin antibodies for use as a diagnostic aid for chronic progressive lymphoedema in Belgian Draught Horses. Vet Immunol Immunopathol. 2015;163:16-22. (ELISA)

4. Colson YL, Li H, Boggs SS, Patrene KD, Johnson PC, Ildstad ST. Durable mixed allogeneic chimerism and tolerance by a nonlethal radiation-based cytoreductive approach. J Immunol. 1996;157:2820-9. (FC)

5. Flynn J, Cox CV, Rizzo S, Foukaneli T, Rice K, Murphy M, et al. Direct binding of antithymoctye globulin to haemopoietic progenitor cells in aplastic anaemia. Br J Haematol. 2003;122:289-97. (FC)

6. Toscan G, Cadore GC, Pereira RC, Silva GB, Cezar AS, Sangioni LA, et al. Equine neosporosis: occurrence of antibodies against Neospora spp. and association between the serological status of the mares and of their offspring. Pesq Vet Bras. 2010;30:641-5. (ICC)

7. van 't Veer C, Mann KG. Regulation of tissue factor initiated thrombin generation by the stoichiometric inhibitors tissue factor pathway inhibitor, antithrombin-III, and heparin cofactor-II. J Biol Chem. 1997;272:4367-77. (WB)

8. Warner S, Hartley CA, Stevenson RA, Ficorilli N, Varrasso A, Studdert MJ, et al. Evidence that Equine rhinitis A virus VP1 is a target of neutralizing antibodies and participates directly in receptor binding. J Virol. 2001;75:9274-81. (WB)

9. Campbell JE, Brummel-Ziedins KE, Butenas S, Mann KG. Cellular regulation of blood coagulation: a model for venous stasis. Blood. 2010;116:6082-91. (WB)

10. Zhang J, Meng W, Wang C, Wu Z, Wu G, Xu Y. Expression, purification and characterization of recombinant plasminogen activator from Gloydius brevicaudus venom in Escherichia coli. Protein Expr Purif. 2013;91:85-90. (WB)










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