Goat Anti-Chicken IgY(H+L)-Alexa Fluor® 488



Datasheet      Tech Support









Antibody Testing Data:

FLISA plate was coated with purified chicken IgY and IgM. Immunoglobulins were detected with Goat Anti-Chicken IgY(H+L)-AF488 (SB Cat. No. 6100-30).








Product Details






Product Specifications

Name:

Goat Anti-Chicken IgY(H+L)-Alexa Fluor® 488

Clone:

-

Isotype:

Goat IgG ( Isotype Control: Goat IgG-Alexa Fluor® 488 )

Specificity:

Reacts with the heavy and light chains of chicken IgY

Format/Conjugate:

AF488 (Alexa Fluor® 488)

Buffer Formulation:

Phosphate buffered saline containing 0.1% sodium azide

Concentration:

1.0 mg/mL

Volume:

1.0 mL

Storage & Handling:

2-8°C; Avoid exposure to light blue Please refer to product specific SDS.

RRID:

AB_2796165

Recommended Dilutions:

Please refer to product specific Technical Guide.

Applications:

Quality tested applications for relevant formats include -
ELISA 1-3,5-7
FLISA
Other referenced applications for relevant formats include -
Flow Cytometry 8-10
Immunohistochemistry-Paraffin Sections 11
Immunocytochemistry 4,5,12-16
Western Blot 1,3,4,17-23

More information:

Source: Pooled antisera from goats hyperimmunized with IgY
Cross Adsorption : None; may react with immunoglobulins from other species and the light chains of other chicken immunoglobulins
Purification: Affinity chromatography on chicken IgY covalently linked to agarose

*For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
















References






1. Chen CH, Lehmeyer JE, Cooper MD. Evidence for an IgD homologue on chicken lymphocytes. J Immunol. 1982;129:2580-5. (Immunogen, IP)

2. Javed MA, Frasca S Jr, Rood D, Cecchini K, Gladd M, Geary SJ, et al. Correlates of immune protection in chickens vaccinated with Mycoplasma gallisepticum strain GT5 following challenge with pathogenic M. gallisepticum strain Rlow. Infect Immun. 2005;73:5410-9. (IHC-FS)

3. Hansell C, Zhu XW, Brooks H, Sheppard M, Withanage S, Maskell D, et al. Unique features and distribution of the chicken CD83+ cell. J Immunol. 2007;179:5117-25. (IHC-FS)

4. Bader SR, Kothlow S, Trapp S, Schwarz SC, Philipp H, Weigend S, et al. Acute paretic syndrome in juvenile White Leghorn chickens resembles late stages of acute inflammatory demyelinating polyneuropathies in humans. J Neuroinflammation. 2010;7:7. (IHC-FS)

5. Singh S, Briles WE, Lupiani B, Collisson EW. Avian influenza viral nucleocapsid and hemagglutinin proteins induce chicken CD8+ memory T lymphocytes. Virology. 2010;399:231-8. (ICC)

6. Bailey JS, Rolón A, Hofacre CL, Holt PS, Wilson JL, Cosby DE, et al. Intestinal humoral immune response and resistance to Salmonella challenge of progeny from breeders vaccinated with killed antigen. Intl J Poult Sci. 2007;6:417-23. (ELISA)

7. Fasina YO, Holt PS, Moran ET, Moore RW, Conner DE, McKee SR. Intestinal cytokine response of commercial source broiler chicks to Salmonella typhimurium infection. Poult Sci. 2008;87:1335-46. (ELISA)

8. Lardinois A, van den Berg T, Lambrecht B, Steensels M. A model for the transfer of passive immunity against Newcastle disease and avian influenza in specific pathogen free chickens. Avian Pathol. 2014;43:118-24. (ELISA)

9. Orr-Burks N, Gulley SL, Gallardo RA, Toro H, van Ginkel FW. Immunoglobulin A as an early humoral responder after mucosal avian coronavirus vaccination. Avian Dis. 2014;58:279-86. (ELISA)

10. Del Cacho E, Gallego M, López-Bernard F, Sánchez-Acedo C, Lillehoj HS. Isolation of chicken follicular dendritic cells. J Immunol Methods. 2008;334:59-69. (FC)

11. Del Cacho E, Gallego M, Lillehoj HS, López-Bernard F, Sánchez-Acedo C. Avian follicular and interdigitating dendritic cells: isolation and morphologic, phenotypic, and functional analyses. Vet Immunol Immunopathol. 2009;129:66-75. (FC)

12. Janardhana V, Broadway MM, Bruce MP, Lowenthal JW, Geier MS, Hughes RJ, et al. Prebiotics modulate immune responses in the gut-associated lymphoid tissue of chickens. J Nutr. 2009;139:1404-9. (FC)

13. Petkov DI, Linnemann EG, Kapczynski DR, Sellers HS. Identification and characterization of two distinct bursal B-cell subpopulations following infectious bursal disease virus infection of White Leghorn chickens. Avian Dis. 2009;53:347-55. (FC)
Alexa Fluor® is a registered trademark of Life Technologies Corporation










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