Biotin Anti-Mouse IgG3 rabbit monoclonal antibody [RM218]
Datasheet
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Catalog#: 31-1094-02
Applications: WB, IP, ICC, IHC, FC, ELISA
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Product Details
Product Specifications |
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|---|---|
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Product Name |
Biotin Anti-Mouse IgG3 rabbit monoclonal antibody [RM218] |
| Product Description: |
Biotinylated Rabbit monoclonal to Mouse IgG3 |
|
Catalog No. |
31-1094-02 |
|
Clone Name |
RM218 |
| Product Datasheet |
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| Specificity |
This antibody reacts to the Fab region of mouse IgG3. No cross reactivity with mouse IgG1, IgG2a, IgG2b, IgM, IgA, IgE, human IgG, or rat IgG. |
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Immunogen |
Mouse IgG3 |
|
Application |
WB, IP, ICC, IHC, FC, ELISA |
|
Species Reactivity |
Mouse |
| Conjugate |
Biotin |
|
Intended Use |
Research Use Only |
Properties |
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|---|---|
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Host |
Rabbit |
|
Storage Instruction |
Store at -20.0°C |
| Stability |
Stable for 1 Year at -20.0°C from date of receipt |
Storage Buffer |
50% Glycerol/PBS with 1% BSA and 0.09% sodium azide |
Concentration |
1 mg/mL |
Volume/Size |
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| Purity |
Protein A affinity purified from an animal origin-free culture supernatant |
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Clone Type |
Recombinant Monoclonal |
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Isotype |
Rabbit IgG |
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Uniport |
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Gene ID |
380795 |
| Usage |
ELISA: 0.005 ug/mL-0.2 ug/mL;Immunocytochemistry (ICC): 0.5 ug/mL-2 ug/mL;Immunohistochemistry (IHC): 0.5 ug/mL-2 ug/mL;Western Blot (WB): 0.1 ug/mL-0.5 ug/mL. |
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Keywords |
- |
*For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Product Images
ELISA of mouse immunoglobulins shows RM218 reacts to both mouse IgG3κ and IgG3λ; No cross reactivity with mouse IgG1, IgG2a, IgG2b, IgM, IgA, IgE, human IgG, or rat IgG. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50 ng/mL, or 10 ng/mL of RM218 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
A titer ELISA of mouse IgG3. The plate was coated with different amounts of mouse IgG3. A serial dilution of RM218 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.