Biotin Anti-Mouse IgG2b rabbit monoclonal antibody [RM108]
Datasheet
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Catalog#: 31-1004-02
Applications: WB (stronger on nonreduced), IP, ICC, IHC, FC, ELISA
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Product Details
Product Specifications |
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|---|---|
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Product Name |
Biotin Anti-Mouse IgG2b rabbit monoclonal antibody [RM108] |
| Product Description: |
Biotinylated Rabbit monoclonal to Mouse IgG2b |
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Catalog No. |
31-1004-02 |
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Clone Name |
RM108 |
| Product Datasheet |
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| Specificity |
This antibody reacts to the Fc region of mouse IgG2b. No cross reactivity with mouse IgG1, IgG2a, IgG3, IgM, IgA, IgE, human IgG, rat IgG, or goat IgG. |
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Immunogen |
Mouse IgG2b |
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Application |
WB (stronger on nonreduced), IP, ICC, IHC, FC, ELISA |
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Species Reactivity |
Mouse |
| Conjugate |
Biotin |
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Intended Use |
Research Use Only |
Properties |
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|---|---|
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Host |
Rabbit |
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Storage Instruction |
Store at -20.0°C |
| Stability |
Stable for 1 Year at -20.0°C from date of receipt |
Storage Buffer |
50% Glycerol/PBS with 1% BSA and 0.09% sodium azide |
Concentration |
1 mg/mL |
Volume/Size |
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| Purity |
Protein A affinity purified from an animal origin-free culture supernatant |
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Clone Type |
Recombinant Monoclonal |
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Isotype |
Rabbit IgG |
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Uniport |
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Gene ID |
16016 |
| Usage |
ELISA: 0.005 ug/mL – 0.2 ug/mL;Immunocytochemistry (ICC): 0.5 ug/mL-2 ug/mL;Immunohistochemistry (IHC): 0.5 ug/mL-2 ug/mL;Western Blot (WB): 0.1 ug/mL-0.5 ug/mL. |
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Keywords |
- |
*For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Product Images
Western blot of nonreduced(-) and reduced(+) mouse IgG2b (20ng/lane), using 0.2ug/mL of RevMAb clone RM108. This antibody reacts to nonreduced IgG2b (~150 kDa) much stronger than the reduced γ2b form (~50 kDa).
ELISA of mouse immunoglobulins shows RM108 reacts to the Fc region of mouse IgG2b; no cross reactivity with IgG1, IgG2a, IgG3, IgM, IgA, IgE, human IgG, rat IgG, or goat IgG. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50 ng/mL, or 10 ng/mL of RM108 was used as the primary antibody. An alkaline phosph- atase conjugated anti-rabbit IgG as the secondary antibody.
A titer ELISA of mouse IgG2b. The plate was coated with different amounts of mouse IgG2b. A serial dilution of RM108 was used as the primary antibody. An alkaline phosphatase conjugated anti- rabbit IgG as the secondary antibody.