Biotin Anti-Mouse IgG2a rabbit monoclonal antibody [RM219]
Datasheet
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Catalog#: 31-1098-02
Applications: WB (nonreduced only), IP, ICC, IHC, FC, ELISA
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Product Details
Product Specifications |
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|---|---|
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Product Name |
Biotin Anti-Mouse IgG2a rabbit monoclonal antibody [RM219] |
| Product Description: |
Biotinylated Rabbit monoclonal to Mouse IgG2a |
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Catalog No. |
31-1098-02 |
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Clone Name |
RM219 |
| Product Datasheet |
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| Specificity |
This antibody reacts to the Fc region of mouse IgG2a. No cross reactivity with mouse IgG1, IgG2b, IgG2c, IgG3, IgM, IgA, IgE, human IgG, or rat IgG. |
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Immunogen |
Mouse IgG2a |
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Application |
WB (nonreduced only), IP, ICC, IHC, FC, ELISA |
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Species Reactivity |
Mouse |
| Conjugate |
Biotin |
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Intended Use |
Research Use Only |
Properties |
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|---|---|
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Host |
Rabbit |
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Storage Instruction |
Store at -20.0°C |
| Stability |
Stable for 1 Year at -20.0°C from date of receipt |
Storage Buffer |
50% Glycerol/PBS with 1% BSA and 0.09% sodium azide |
Concentration |
1 mg/mL |
Volume/Size |
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| Purity |
Protein A affinity purified from an animal origin-free culture supernatant |
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Clone Type |
Recombinant Monoclonal |
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Isotype |
Rabbit IgG |
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Uniport |
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Gene ID |
380793 |
| Usage |
ELISA: 0.005 ug/mL – 0.2 ug/mL;Immunocytochemistry (ICC): 0.5 ug/mL-2 ug/mL;Immunohistochemistry (IHC): 0.5 ug/mL-2 ug/mL;Western Blot (WB): 0.5 ug/mL-2 ug/mL. |
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Keywords |
- |
*For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Product Images
ELISA of mouse immunoglobulins shows RM219 reacts to the Fc region of mouse IgG2a; no cross reactivity with IgG1, IgG2b, IgG2c, IgG3, IgM, IgA, IgE, human IgG, or rat IgG. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50 ng/mL, or 10 ng/mL of RM219 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
A titer ELISA of mouse IgG2a. The plate was coated with different amounts of mouse IgG2a. A serial dilution of RM219 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
Western blot of nonreduced(-) and reduced(+) mouse IgG2a, using 0.5ug/mL of RevMAb clone RM219. This antibody reacts to nonreduced IgG2a (~150 kDa).