Biotin Anti-Mouse IgG2a kappa rabbit monoclonal antibody [RM107]
Datasheet
Tech SupportView All Antibodies
Catalog#: 31-1003-02
Applications: WB (nonreduced only), IP, ICC, IHC, FC, ELISA
Price Not Available
Request bulk or custom quote
Product Details
Product Specifications |
|
|---|---|
|
Product Name |
Biotin Anti-Mouse IgG2a kappa rabbit monoclonal antibody [RM107] |
| Product Description: |
Biotinylated Rabbit monoclonal to Mouse IgG2a Kappa |
|
Catalog No. |
31-1003-02 |
|
Clone Name |
RM107 |
| Product Datasheet |
Biotin Anti-Mouse IgG2a kappa rabbit monoclonal antibody [RM107] |
| Specificity |
This antibody reacts to the Fab region of mouse IgG2ak. No cross reactivity with mouse IgG2al, IgG1, IgG3, IgM, IgA, IgE, human IgG, rat IgG, or goat IgG. |
|
Immunogen |
Mouse IgG |
|
Application |
WB (nonreduced only), IP, ICC, IHC, FC, ELISA |
|
Species Reactivity |
Mouse |
| Conjugate |
Biotin |
|
Intended Use |
Research Use Only |
Properties |
|
|---|---|
|
Host |
Rabbit |
|
Storage Instruction |
Store at -20.0°C |
| Stability |
Stable for 1 Year at -20.0°C from date of receipt |
Storage Buffer |
50% Glycerol/PBS with 1% BSA and 0.09% sodium azide |
Concentration |
1 mg/mL |
Volume/Size |
Biotin Anti-Mouse IgG2a kappa rabbit monoclonal antibody [RM107] |
| Purity |
Protein A affinity purified from an animal origin-free culture supernatant |
|
Clone Type |
Recombinant Monoclonal |
|
Isotype |
Rabbit IgG |
|
Uniport |
|
|
Gene ID |
380793 |
| Usage |
ELISA: 0.005 ug/mL – 0.2 ug/mL;Immunocytochemistry (ICC): 0.5 ug/mL-2 ug/mL;Immunohistochemistry (IHC): 0.5 ug/mL-2 ug/mL;Western Blot (WB): 0.1 ug/mL-0.5 ug/mL. |
|
Keywords |
- |
*For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Product Images
Western blot of nonreduced(-) and reduced(+) mouse IgG2aκ (20 ng/lane), using 0.2ug/mL of RevMAb clone RM107. This antibody only reacts to nonreduced Mouse IgG2aκ.
ELISA of mouse immunoglobulins shows RM107 reacts to the Fab region of mouse IgG2aκ; no cross reactivity with IgG2aλ, IgG1, IgG3, IgM, IgA, IgE, human IgG, rat IgG, or goat IgG. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50 ng/mL, or 10 ng/mL of RM107 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
A titer ELISA of mouse IgG2aκ. The plate was coated with different amounts of mouse IgG2aκ. A serial dilution of RM107 was used as the primary antibody. An alkaline phosphatase conjugated anti- rabbit IgG as the secondary antibody.