Biotin Anti-Mouse IgG1 rabbit monoclonal antibody [RM106]
Datasheet
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Catalog#: 31-1002-02
Applications: WB, IP, ICC, IHC, FC, ELISA
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Product Details
Product Specifications |
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Product Name |
Biotin Anti-Mouse IgG1 rabbit monoclonal antibody [RM106] |
| Product Description: |
Biotinylated Rabbit monoclonal to Mouse IgG1 |
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Catalog No. |
31-1002-02 |
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Clone Name |
RM106 |
| Product Datasheet |
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| Specificity |
This antibody reacts to the Fc region of mouse IgG1. No cross reactivity with mouse IgG2a, IgG3, IgM, IgA, IgE, human IgG, or rat IgG. It may slightly cross react to mouse IgG2b, and may also react to goat IgG. |
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Immunogen |
Mouse IgG |
|
Application |
WB, IP, ICC, IHC, FC, ELISA |
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Species Reactivity |
Mouse |
| Conjugate |
Biotin |
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Intended Use |
Research Use Only |
Properties |
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|---|---|
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Host |
Rabbit |
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Storage Instruction |
Store at -20.0°C |
| Stability |
Stable for 1 Year at -20.0°C from date of receipt |
Storage Buffer |
50% Glycerol/PBS with 1% BSA and 0.09% sodium azide |
Concentration |
1 mg/mL |
Volume/Size |
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| Purity |
Protein A affinity purified from an animal origin-free culture supernatant |
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Clone Type |
Recombinant Monoclonal |
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Isotype |
Rabbit IgG |
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Uniport |
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Gene ID |
16017 |
| Usage |
ELISA: 0.005 ug/mL-0.2 ug/mL;Immunocytochemistry (ICC): 0.5 ug/mL-2 ug/mL;Immunohistochemistry (IHC): 0.5 ug/mL-2 ug/mL;Western Blot (WB): 0.1 ug/mL-0.5 ug/mL. |
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Keywords |
- |
*For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Product Images
Western blot of nonreduced(-) and reduced(+) mouse IgG1 (20ng/lane), using 0.2ug/mL of RevMAb clone RM106. This antibody reacts to nonreduced IgG1 (~150 kDa) stronger than the reduced γ1 form (~50 kDa).
ELISA of mouse immunoglobulins shows RM106 reacts to the Fc region of mouse IgG1; no cross reactivity with IgG2a, IgG3, IgM, IgA, IgE, human IgG, or rat IgG. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50 ng/mL, or 10 ng/mL of RM106 was used as the primary antibody. An alkaline phosphatase conjugated anti- rabbit IgG as the secondary antibody.
A titer ELISA of mouse IgG1. The plate was coated with different amount of mouse IgG1. A serial dilution of RM106 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.