Biotin Anti-Human IgG4 Fc rabbit monoclonal antibody [RM217]
Datasheet Tech Support
View All Antibodies
Product Details
Product Specifications |
|
---|---|
Product Name |
Biotin Anti-Human IgG4 Fc rabbit monoclonal antibody [RM217] |
Product Description: |
Biotinylated Rabbit monoclonal to Human IgG4 Fc |
Catalog No. |
31-1100-02 |
Clone Name |
RM217 |
Product Datasheet |
Biotin Anti-Human IgG4 Fc rabbit monoclonal antibody [RM217] |
Specificity |
This antibody reacts to the Fc region of human IgG4. No cross reactivity with human IgG1, IgG2, IgG3, IgM, IgA, IgD, IgE, mouse IgG, rat IgG, or goat IgG. |
Immunogen |
Peptide corresponding to the Fc region of Human IgG4 |
Application |
FC, ELISA |
Species Reactivity |
Human |
Conjugate |
Biotin |
Intended Use |
Research Use Only |
Properties |
|
---|---|
Host |
Rabbit |
Storage Instruction |
Store at -20.0°C |
Stability |
Stable for 1 Year at -20.0°C from date of receipt |
Storage Buffer |
50% Glycerol/PBS with 1% BSA and 0.09% sodium azide |
Concentration |
1 mg/mL |
Volume/Size |
Biotin Anti-Human IgG4 Fc rabbit monoclonal antibody [RM217] |
Purity |
Protein A affinity purified from an animal origin-free culture supernatant |
Clone Type |
Recombinant Monoclonal |
Isotype |
Rabbit IgG |
Uniport |
|
Gene ID |
3503 |
Usage |
ELISA: 50ng/well – 200ng/well (for Capture);0.05ug/mL – 0.2ug/mL (for Detection). |
Keywords |
- |
*For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Product Images
RM217 specifically reacts to human IgG4. No cross reactivity with human IgG1, IgG2, IgG3.
Sandwich ELISA using RM217 as the capture antibody, and Biotinylated anti-human light chains ( κ+ λ) antibody RM129 as the detection antibody, followed by an AP conjugated streptavidin.
A titer ELISA using RM217. The plate was coated with different amounts of human IgG4. A serial dilution of RM217 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.