Biotin Anti-Human IgG2 rabbit monoclonal antibody [RM118]
Datasheet
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Catalog#: 31-1020-02
Applications: ICC, IHC, FC, ELISA
Price Not Available
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Product Details
Product Specifications |
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|---|---|
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Product Name |
Biotin Anti-Human IgG2 rabbit monoclonal antibody [RM118] |
| Product Description: |
Biotinylated Rabbit monoclonal to Human IgG2 |
|
Catalog No. |
31-1020-02 |
|
Clone Name |
RM118 |
| Product Datasheet |
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| Specificity |
This antibody reacts to the heavy chain of human IgG2. No cross reactivity with human IgG1, IgG3, IgG4, IgM, IgA, IgD, IgE, mouse IgG, rat IgG, or goat IgG. |
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Immunogen |
Human IgG2 |
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Application |
ICC, IHC, FC, ELISA |
|
Species Reactivity |
Human |
| Conjugate |
Biotin |
|
Intended Use |
Research Use Only |
Properties |
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|---|---|
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Host |
Rabbit |
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Storage Instruction |
Store at -20.0°C |
| Stability |
Stable for 1 Year at -20.0°C from date of receipt |
Storage Buffer |
50% Glycerol/PBS with 1% BSA and 0.09% sodium azide |
Concentration |
1 mg/mL |
Volume/Size |
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| Purity |
Protein A affinity purified from an animal origin-free culture supernatant |
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Clone Type |
Recombinant Monoclonal |
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Isotype |
Rabbit IgG |
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Uniport |
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Gene ID |
3501 |
| Usage |
ELISA: 50ng/well – 200ng/well (for Capture);0.05ug/mL – 0.2ug/mL (for Detection);Immunocytochemistry (ICC): 0.5ug/mL-2 ug/mL;Immunohistochemistry (IHC): 0.5 ug/mL-2 ug/mL. |
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Keywords |
- |
*For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Product Images
Sandwich ELISA using RM118 as the capture antibody, and Biotinylated anti-human light chains ( κ+ λ) antibody RM129 as the detection antibody, followed by an AP conjugated streptavidin.
Sandwich ELISA using RM118 as the capture antibody, and Biotinylated anti-human light chains ( κ+ λ) antibody RM129 as the detection antibody, followed by an AP conjugated streptavidin.
ELISA of human immunoglobulins shows RM118 only reacted to human IgG2. No cross reactivity with Human IgG1, IgG3, IgG4, IgE, IgD, IgA, mouse IgG, rat IgG, or goat IgG. The plate was coated with 50 ng/well of different immunoglobulins. A serial dilution of RM118 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.