Catalog#: 31-1024-02
Applications: ICC, IHC, FC, ELISA
Price Not Available
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Product Details
Product Specifications |
|
---|---|
Product Name |
Biotin Anti-Human IgE rabbit monoclonal antibody [RM122] |
Product Description: |
Biotinylated Rabbit monoclonal to Human IgE |
Catalog No. |
31-1024-02 |
Clone Name |
RM122 |
Product Datasheet |
|
Specificity |
This antibody reacts to human IgE. No cross reactivity with human IgG, IgM, IgD, or IgA. |
Immunogen |
Human IgE |
Application |
ICC, IHC, FC, ELISA |
Species Reactivity |
Human |
Conjugate |
Biotin |
Intended Use |
Research Use Only |
Properties |
|
---|---|
Host |
Rabbit |
Storage Instruction |
Store at -20.0°C |
Stability |
Stable for 1 Year at -20.0°C from date of receipt |
Storage Buffer |
50% Glycerol/PBS with 1% BSA and 0.09% sodium azide |
Concentration |
1 mg/mL |
Volume/Size |
|
Purity |
Protein A affinity purified from an animal origin-free culture supernatant |
Clone Type |
Recombinant Monoclonal |
Isotype |
Rabbit IgG |
Uniport |
|
Gene ID |
3497 |
Usage |
ELISA: 10ng/well – 100ng/well (for Capture);0.01ug/mL – 0.1ug/mL (for Detection);Immunocytochemistry (ICC): 0.5ug/mL-2ug/mL;Immunohistochemistry (IHC): 0.5ug/mL-2ug/mL. |
Keywords |
- |
*For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Product Images
Sandwich ELISA using RM122 as the capture antibody (25ng/well), and Biotinylated anti-human light chains ( κ+ λ) antibody RM129 as the detection antibody, followed by an alkaline phosphatase conjugated streptavidin.
ELISA of human immunoglobulins shows RM122 reacts only to human IgEλ from human myeloma plasma and the IgE from human plasma. No cross reactivity with Human IgG, IgM, IgD, or IgA. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50 ng/mL or 10 ng/mL of RM122 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
A titer ELISA using RM122. The plate was coated with different amounts of human IgE. A serial dilution of RM122 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.