Catalog#: 31-1175-00-S/L
Applications: WB, ELISA, IHC/ICC
Price Not Available
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Product Details
Product Specifications |
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|---|---|
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Product Name |
Anti-Histone H3 K27M rabbit monoclonal antibody [RM192] |
| Product Description: |
Rabbit monoclonal to K27M Histone H3 |
|
Catalog No. |
31-1175-00-S/L |
|
Clone Name |
RM192 |
| Product Datasheet |
|
| Specificity |
This antibody reacts to the Histone H3 K27M mutant. No cross reactivity with wild type Histone H3. |
|
Immunogen |
A peptide corresponding to Histone H3 K27M mutant |
|
Application |
WB, ELISA, IHC/ICC |
|
Species Reactivity |
Human |
| Conjugate |
None |
|
Intended Use |
Research Use Only |
Properties |
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|---|---|
|
Host |
Rabbit |
|
Storage Instruction |
Store at -20.0°C |
| Stability |
Stable for 1 Year at -20.0°C from date of receipt |
Storage Buffer |
50% Glycerol/PBS with 1% BSA and 0.09% sodium azide |
Concentration |
N/A |
Volume/Size |
|
| Purity |
Protein A affinity purified from an animal origin-free culture supernatant |
|
Clone Type |
Recombinant Monoclonal |
|
Isotype |
Rabbit IgG |
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Uniport |
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|
Gene ID |
8350 |
| Usage |
Western Blot: 1:250 – 1:2500;ELISA: 1:250 – 1:2500;IHC: 1:100 – 1:500. |
|
Keywords |
- |
*For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Product Images
Immunostaining of brain tumor tissue sections using both EDTA and Citrate pretreatment methods. Clinically validated to be Positive for the H3.3 K27M Mutation by Sanger sequencing.
Image courtesy of Sebastian Brandner MD, Division of Neuropathology and Dept. of Neurodegenerative Disease, UCL Institute of Neurology, London, UK
Immunostaining of brain tumor tissue sections using both EDTA and Citrate pretreatment methods. Clinically validated to be Negative for the H3.3 K27M Mutation by Sanger sequencing.
Image courtesy of Sebastian Brandner MD, Division of Neuropathology and Dept. of Neurodegenerative Disease, UCL Institute of Neurology, London, UK
Western Blot analysis of cell lysates prepared from 293T transfected with a DNA construct encoding wild type (WT) or K27M mutant proteins of Histone H3.3, using 0.01 ug/mL of anti-Histone H3 K27M rabbit monoclonal antibody clone RM192.