Catalog#: 31-1048-00
Applications: WB, IP, ICC, FC , IHC
Price Not Available
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Product Details
Product Specifications |
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|---|---|
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Product Name |
Anti-His-Tag rabbit monoclonal antibody [RM146] |
| Product Description: |
Rabbit monoclonal to His-Tag (6xHis-Tag or 10xHis-Tag) |
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Catalog No. |
31-1048-00 |
|
Clone Name |
RM146 |
| Product Datasheet |
|
| Specificity |
This antibody reacts to recombinant proteins containing the 6xHis-Tag or 10xHis-Tag fused to either the amino or carboxy terminus. No cross reactivity with other endogenous protein in mammalian or bacteria cells. |
|
Immunogen |
Mixture of a peptide with 6xHis-Tag at the N-terminus and another peptide with 6xHis-Tag at the C-terminus. |
|
Application |
WB, IP, ICC, FC , IHC |
|
Species Reactivity |
All Species |
| Conjugate |
None |
|
Intended Use |
Research Use Only |
Properties |
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|---|---|
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Host |
Rabbit |
|
Storage Instruction |
Store at -20.0°C |
| Stability |
Stable for 1 Year at -20.0°C from date of receipt |
Storage Buffer |
50% Glycerol/PBS with 1% BSA and 0.09% sodium azide |
Concentration |
1 mg/mL |
Volume/Size |
|
| Purity |
Protein A affinity purified from an animal origin-free culture supernatant |
|
Clone Type |
Recombinant Monoclonal |
|
Isotype |
Rabbit IgG |
|
Uniport |
|
|
Gene ID |
N/A |
| Usage |
Western Blot: 0.1 ug/mL – 1 ug/mL;Immunoprecipitation: 0.5 ug/mL – 2 ug/mL;Immunocytochemistry 0.5 ug/mL – 2 ug/mL;Flow Cytometry: 0.5 ug/mL – 2 ug/mL;Immunohistochemistry; 0.1 ug/mL-1 ug/mL. |
|
Keywords |
- |
*For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Product Images
Western blot of 293T cells:
(1) untransfected or
(2) transfected with His-Tag fusion protein X and
(3) E. coli lysate without His-Tag Protein Y
(4) E. coli lysate with His-Tag Protein Y
Using RevMAb Clone RM146 at 0.2 ug/mL.
Immunoprecipitation of 293T cells expressing a His-Tag fusion protein by RevMAb clone RM146, was blotted with RM146. (1) Whole lysate control; (2) IP by rabbit IgG control; (3) IP by RM146.
Immunohistochemistry staining of naïve HepG2 cells (Negative control) using anti-His-Tag antibody, RM146.