Catalog#: 54-1191-00
Applications: ELISA (Capture or detector), WB
Price Not Available
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Product Details
Product Specifications |
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|---|---|
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Product Name |
Anti-HA-Tag Chimeric Human Monoclonal Antibody [RMH02] |
| Product Description: |
Chimeric Human monoclonal to HA-Tag |
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Catalog No. |
54-1191-00 |
|
Clone Name |
RMH02 |
| Product Datasheet |
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| Specificity |
This antibody reacts to recombinant proteins containing a HA-Tag fused to either the amino or carboxy terminus. No cross reactivity with any endogenous protein. |
|
Immunogen |
A peptide corresponding to HA-tag (Human influenza hemagglutinin amino acids 98-106). |
|
Application |
ELISA (Capture or detector), WB |
|
Species Reactivity |
N/A |
| Conjugate |
None |
|
Intended Use |
Research Use Only |
Properties |
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|---|---|
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Host |
Rabbit |
|
Storage Instruction |
Store at -20.0°C |
| Stability |
Stable for 1 Year at -20.0°C from date of receipt |
Storage Buffer |
50% Glycerol/PBS with 1% BSA and 0.09% sodium azide |
Concentration |
1 mg/mL |
Volume/Size |
|
| Purity |
Protein A affinity purified from an animal origin-free culture supernatant |
|
Clone Type |
Recombinant Monoclonal |
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Isotype |
Chimeric |
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Uniport |
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|
Gene ID |
N/A |
| Usage |
ELISA: Capturer: 0. 5 ug/mL – 2 ug/mL; Detector: 0.01 ug/mL – 0.5 ug/mL;Western Blot: 0.1 ug/mL – 1 ug/mL. |
|
Keywords |
- |
*For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Product Images
Western blot of 293T cells transfected (+) or non- transfected (-) with a DNA construct encoding HA- Tag KRas fusion protein, using anti-HA-tag rabbit monoclonal antibody (clone RM305) at 0.1 ug/mL, followed by a HRP conjugated anti-Human IgG secondary antibody.
Sandwich ELISA performed on whole lysate of LNCaP cells overexpressing HA-tag Bag1 protein. ELISA plate was coated with 50uL/well of chimeric human anti-HA- Tag antibody Clone RMH02 (1ug/mL) as the capture antibody. Anti-Bag1 rabbit serum was used as the detection antibody, followed by an alkaline phosphatase conjugated anti-Rabbit IgG as the Secondary antibody.