Anti-Acetyl-Histone H3 (Lys18) rabbit monoclonal antibody [RM166]
Datasheet
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Catalog#: 31-1055-00
Applications: WB, ELISA, Multiplex, ChIP, ICC. IHC
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Product Details
Product Specifications |
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|---|---|
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Product Name |
Anti-Acetyl-Histone H3 (Lys18) rabbit monoclonal antibody [RM166] |
| Product Description: |
Rabbit monoclonal to Acetylated Histone H3 Lysine 18 (K18ac) |
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Catalog No. |
31-1055-00 |
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Clone Name |
RM166 |
| Product Datasheet |
Anti-Acetyl-Histone H3 (Lys18) rabbit monoclonal antibody [RM166] |
| Specificity |
This antibody reacts to Histone H3 acetylated at Lysine 18 (K18ac). No cross reactivity with other acetylated Lysines in histone H3. |
|
Immunogen |
An acetyl-peptide corresponding to the Acetyl-Histone H3 (Lys18) |
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Application |
WB, ELISA, Multiplex, ChIP, ICC. IHC |
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Species Reactivity |
Human Vertebrates |
| Conjugate |
None |
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Intended Use |
Research Use Only |
Properties |
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Host |
Rabbit |
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Storage Instruction |
Store at -20.0°C |
| Stability |
Stable for 1 Year at -20.0°C from date of receipt |
Storage Buffer |
50% Glycerol/PBS with 1% BSA and 0.09% sodium azide |
Concentration |
1 mg/mL |
Volume/Size |
Anti-Acetyl-Histone H3 (Lys18) rabbit monoclonal antibody [RM166] |
| Purity |
Protein A affinity purified from an animal origin-free culture supernatant |
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Clone Type |
Recombinant Monoclonal |
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Isotype |
Rabbit IgG |
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Uniport |
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Gene ID |
8350 |
| Usage |
Western Blot: 0.5 ug/mL - 2 ug/mL;ICC: 0.5 ug/mL - 2 ug/mL;ChIP: 2 ug/mL-10 ug/mL;IHC: 1 ug/mL-10 ug/mL;ELISA: 0.2 ug/mL - 1 ug/mL;Multiplex: 0.1 ug/mL – 0.5 ug/mL. |
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Keywords |
- |
*For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Product Images
Immunohistochemistry staining of HepG2 cells using Anti-Acetyl-Histone H3 (Lys18) antibody, RM166.
ChIP performed on HeLa cells with or without Sodium Butyrate treatment, using H3K18Ac antibody (RM166, 5ug). Real-time PCR was performed using primers specific to the gene indicated.
RM166 specifically reacts to Histone H3 acetylated at Lysine 18 (K18ac). No cross reactivity with acetylated Lysine 4 (K4ac), Lysine 9 (K9ac), Lysine 14 (K14ac), Lysine 23 (K23ac), Lysine 27 (K27ac), Lysine 36 (K36ac), Lysine 56 (K56ac), Lysine 79 (K79ac), or Lysine 122 (K122) in Histone H3.