Anti-5-methylcytosine (5-mC) rabbit monoclonal antibody [RM231]
Datasheet
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Catalog#: 31-1110-00
Applications: DB, MeDIP, ICC, IHC, ELISA, FC
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Product Details
Product Specifications |
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Product Name |
Anti-5-methylcytosine (5-mC) rabbit monoclonal antibody [RM231] |
| Product Description: |
Rabbit monoclonal to 5-methylcytosine (5-mC) Rabbit Monoclonal Antibody |
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Catalog No. |
31-1110-00 |
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Clone Name |
RM231 |
| Product Datasheet |
Anti-5-methylcytosine (5-mC) rabbit monoclonal antibody [RM231] |
| Specificity |
This antibody reacts to 5-methylcytosine in both single-stranded and double-stranded DNA. No cross reactivity with non-methylated cytosine and hydroxymethylcytosine in DNA. |
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Immunogen |
BSA-conjugated 5-methylcytosine. |
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Application |
DB, MeDIP, ICC, IHC, ELISA, FC |
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Species Reactivity |
All Vertebrates |
| Conjugate |
None |
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Intended Use |
Research Use Only |
Properties |
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Host |
Rabbit |
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Storage Instruction |
Store at -20.0°C |
| Stability |
Stable for 1 Year at -20.0°C from date of receipt |
Storage Buffer |
50% Glycerol/PBS with 1% BSA and 0.09% sodium azide |
Concentration |
1 mg/mL |
Volume/Size |
Anti-5-methylcytosine (5-mC) rabbit monoclonal antibody [RM231] |
| Purity |
Protein A affinity purified from an animal origin-free culture supernatant |
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Clone Type |
Recombinant Monoclonal |
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Isotype |
Rabbit IgG |
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Uniport |
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Gene ID |
N/A |
| Usage |
Dot Blot: 0.5 ug/mL – 2 ug/mL;ELISA: 0.1 ug/mL - 1 ug/mL;ICC: 0.5 ug/mL - 2 ug/mL. |
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Keywords |
- |
*For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Product Images
ELISA of single stranded DNA using rabbit monoclonal anti-5-mC (clone RM231) antibody. The plate was coated with streptavidin and then biotinylated single stranded unmethylated DNA, 5-Methylcytosine (5-mC) DNA, and 5-Hydroxymethylcytosine (5-hmC) DNA. A serial dilution of RM231 was used as the primary antibody, and an alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
Dot blot of double stranded DNA using rabbit monoclonal anti-5-mC (clone RM231) antibody. The membrane was pre-spotted with 50, 5, and 0.5 ng/dot of double stranded 5-Hydroxymethylcytosine (5-hmC) DNA, 5-Methylcytosine (5-mC) DNA, and unmethylated DNA. The pre-spotted membrane was then blotted with RM231.
Immunocytochemical staining of HeLa cells using rabbit monoclonal anti-5-mC (clone RM231) antibody (red). Actin filaments have been labeled with fluorescein phalloidin (green), and nuclei stained with DAPI (blue).