Anti 5-hydroxymethylcytosine (5-hmC) Rabbit Monoclonal Antibody [RM236]
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Catalog#: 31-1111-00
Applications: DB, MeDIP, ICC, IHC, ELISA, FC
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Product Details
Product Specifications |
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Product Name |
Anti 5-hydroxymethylcytosine (5-hmC) Rabbit Monoclonal Antibody [RM236] |
Product Description: |
Rabbit monoclonal to 5-hydroxymethylcytosine (5-hmC); 5'-hmC |
Catalog No. |
31-1111-00 |
Clone Name |
RM236 |
Product Datasheet |
Anti 5-hydroxymethylcytosine (5-hmC) Rabbit Monoclonal Antibody [RM236] |
Specificity |
This antibody reacts to 5-hydroxymethylcytosine in both single-stranded and double-stranded DNA. No cross reactivity with non-methylated cytosine and methylcytosine in DNA. |
Immunogen |
BSA-conjugated 5-hydroxymethylcytosine. |
Application |
DB, MeDIP, ICC, IHC, ELISA, FC |
Species Reactivity |
All Vertebrates |
Conjugate |
None |
Intended Use |
Research Use Only |
Properties |
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Host |
Rabbit |
Storage Instruction |
Store at -20.0°C |
Stability |
Stable for 1 Year at -20.0°C from date of receipt |
Storage Buffer |
50% Glycerol/PBS with 1% BSA and 0.09% sodium azide |
Concentration |
1 mg/mL |
Volume/Size |
Anti 5-hydroxymethylcytosine (5-hmC) Rabbit Monoclonal Antibody [RM236] |
Purity |
Protein A affinity purified from an animal origin-free culture supernatant |
Clone Type |
Recombinant Monoclonal |
Isotype |
Rabbit IgG |
Uniport |
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Gene ID |
N/A |
Usage |
Dot Blot: 0.2 ug/mL – 1 ug/mL;ELISA: 0.1 ug/mL - 1 ug/mL;IHC: 0.1 ug/mL - 1 ug/mL. |
Keywords |
- |
*For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Product Images
ELISA of single stranded DNA using rabbit monoclonal anti-5-hmC (clone RM236) antibody. The plate was coated with streptavidin and then biotinylated single stranded unmethylated DNA, 5-Methylcytosine (5-mC) DNA, and 5-Hydroxymethylcytosine (5-hmC) DNA. A serial dilution of RM236 was used as the primary antibody, and an alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
Dot blot of double stranded DNA using rabbit monoclonal anti-5-hmC (clone RM236) antibody. The membrane was pre-spotted with 50, 5, and 0.5 ng/dot of double stranded 5-Hydroxymethylcytosine (5-hmC) DNA, 5-Methylcytosine (5-mC) DNA, and unmethylated DNA. The pre-spotted membrane was then blotted with RM236.
Immunohistochemical staining of formalin fixed and paraffin embedded human brain tissue sections, using rabbit monoclonal anti-5-hmC (clone RM236) antibody.