- Low cross-reactivity with cytosine and 5-methylcytosine versus other available antibodies.
- Can be used with a variety of genomic DNA sources.
- High sensitivity to low masses of 5-hydroxymethylcytosine DNA.
The rabbit Anti-5-hmC Polyclonal Antibody can robustly distinguish between hydroxymethylated DNA and methylated or unmodified DNA with limited to no cross-reactivity. The antibody has been validated in ELISA and immunoprecipitation-based enrichment assays, and is suitable for use in further applications including immunohistochemical labeling and chromatographic blotting.
|Buffer Solution||Antibody is provided in PBS (Phosphate Buffered Saline, pH 7.4) with 0.05% Sodium azide as a preservative.|
|Protein Concentration||1 mg/ml|
|Specificity||5-hydroxymethylcytosine in single-stranded DNA from any mammalian, plant, insect, or microbial sources, as well as artificial templates used for standardization.|
|Storage||Store at -20°C with prolonged storage at -80° C. Avoid repeated freeze/thawing.|
Anti-5-Hydroxymethylcytosine Polyclonal Antibody
A4001-200 / A4001-25 / A4001-50