RNA Preparation and Purification

Molecular Research Center, Inc., founded in 1989 by Piotr Chomczynski, develops and manufactures innovative products for the isolation and analysis of RNA and DNA. It is a corporation ” run by scientists for scientists” and we are continuously working on the development and improvement of molecular biology techniques.

Beginning with the single-step method for RNA isolation we have worked to simplify nucleic acid isolation protocols while providing improved recovery and sample purity. This method has resulted in the most cited scientific publication in the last 25 years.

Our patented RNA and DNA extraction techniques are recognized around the world as having the best performance, reliability and cost effectiveness.

Products

TRI Reagent® (TR 118) TRI Reagent® is used for RNA isolation from tissues, pelleted cells and cells grown in monolayer. Fifty milliliters is sufficient to process 50 samples, each containing 50 – 100 mg of tissue. Expected yields range from 50 – 700 μg of RNA per sample, depending upon the tissue source.

DNA Extraction Solution (GT 192) After using RNAzol® BD to isolate RNA from blood samples, use DNA Extraction Solution to isolate genomic DNA from the remaining organic phase of RNAzol® BD. Simultaneously isolate RNA and DNA from the same sample using RNAzol® BD and DNA Extraction Solution.

RNAzol® BD Column Kit The RNAzol® BD Column Kit combines RNAzol® BD, with a versatile and universal column in one procedure. This Kit provides unsurpassed yields of either total RNA, large RNA (> 150 – 200 bases) or small RNA (< 150 – 200 bases) in separate fractions. RNAzol® BD has been used to determine that RNA averages 14 μg/ml of human whole blood and ranges from 6 – 22 μg/ml. These RNA levels are significantly higher than previously reported with other methods that average only 2 – 5 μg of total RNA per ml of human blood

RNAzol® BD (RB 192) RNAzol® BD is the most effective reagent for isolation of total RNA from whole blood, plasma or serum of human or animal origin. This patented reagent(1) employs an improved single-step method to provide the highest yield and purity of isolated RNA. RNAzol® BD isolates pure and undegraded RNA that is ready for RT-PCR without DNase treatment (2).

RNAzol® RT Column Kit RNAzol® RT is the most effective reagent for isolation of total RNA and small RNA from samples of human, animal, plant, bacterial and viral origin. This patented reagent(1) provides higher yield and quality of isolated RNA than previous reagents based on the single-step method. RNAzol® RT isolates pure and undegraded RNA that is ready for RT-PCR without DNase treatment.(2)

RNAzol® RT (RN 190) RNAzol® RT is the most effective reagent for isolation of total RNA and small RNA from samples of human, animal, plant, bacterial and viral origin. This patented reagent(1) provides higher yield and quality of isolated RNA than previous reagents based on the single-step method. RNAzol® RT isolates pure and undegraded RNA that is ready for RT-PCR without DNase treatment.(2)