Dual Labeled Fluorescent Probes

Dual Labeled Fluorescent Probes are DNA oligonucleotides of 20-30 bp carrying a fluorophore (5'-end) and a quencher (3'-end). The labeled probe hybridizes sequence-specifically to its complementary section of the amplicon. During DNA extension of each PCR cycle, the fluorophore reporter is being cleaved and released. The resulting, detectable fluorescence signal is proportional to the amount of accumulated PCR product. All labeled probes are purified by HPLC and quality checked by MALDI-TOF. Select from Jena Bioscience’s extensive reporter/quencher repertoire or inquire for alternative combinations

BHQ-1 Quencher    BBQ®-650 Quencher
BHQ-2 Quencher    ECLIPSE Quencher
BHQ-3 Quencher    DABCYL Quencher TAMRA Quencher    


Please select your desired Fluorescent Probe by clicking the basket and type the related sequence into the pop-up window. Prices include up to 40 bases per probe, HPLC purification and MALDI ToF quality check to provide probes of exceptional purity. For longer oligonucleotides or other reporter/quencher combinations please inquire.

A yield of 1 OD represents approx. 33 µg of single-stranded DNA with an equal distribution of the four bases. This corresponds to approx. 5 nmol (50 µl / 100 µM) of a DNA probe consisting of 20 bases.

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