A key tool in understanding protein trafficking is subcellular fractionation. Traditionally, this has been performed by sucrose gradient ultracentrifugation. Unfortunately, not every lab has an ultracentrifuge readily accessible to it. Invent Biotechnologies Minute™ subcellular fractionation kits eliminate the need for ultracentrifugation and make the entire protocol much easier. Following simple, well-defined protocols and using a convenient spin column format, you can obtain preparations of various organelles or other subcellular fractions in under an hour! Cell Fractionation / Protein Trafficking

SPIN-COLUMN-BASED PROTEIN EXTRACTION / CELL FRACTIONATION

Founded in 2010, Invent Biotechnologies is recognized worldwide for having revolutionized protein extraction and cell fractionation. As the inventor of a range of spin column-based disruptive technologies for protein research, we offer a simpler, smarter approach to sample preparation. This translates to streamlined workflows and more consistent results, providing superior data quality. By choosing our Minute™ Kits for protein extraction and cell fractionation, researchers can be confident that their samples accurately represent the endogenous profile of the cells or tissue under investigation. Requiring only a benchtop centrifuge, Minute™ Kits minimize experimental variables and deliver uncompromised material for more reliable data. The success of the Minute™ approach is evidenced by a growing number of publications, including citations in Nature, Science and Cell.

The Minute™ Benefits:
• Super-fast (as quick as 1 minute)
• Small sample size
• Complete profile
• Unaltered endogenous baseline
• All living organism and tissue

The Minute™ Applications:
• Protein trafficking/translocation
• Protein-protein interaction
• Protein modification
• Signal transduction
• Daily protein analysis

Minute™ Nuclear Envelope Protein Extraction Kit (50 Preps) (Invent Biotechnologies, Inc.)

The nuclear envelope is a very complex membrane-protein system that is notoriously difficult to isolate and purify because of its connection to nuclear and cytoplasmic components. Traditional methods of nuclear envelope isolation and purification require a large amount of starting material and a long, tedious procedure.

Minute™ Cytosolic and Nuclear Extraction Kit for Frozen/Fresh Tissues (20 Preps) (Invent Biotechnologies, Inc.)

Preparation of cytosolic and nuclear fractions from cultured cells and tissues is common. Cell fractionation from cultured cells is relatively easy and straightforward. However, a clear separation of cytosolic and nuclear fractions from tissues,..

Minute™ Detergent-Free Nuclear Matrix Protein Extraction Kit (For Mammalian Cultured Cells/Tissues) (20 Preps) (Invent Biotechnologies, Inc.)

Nucleoplasm, also referred to as karyoplasm/nucleus sap, consists of chromosomes, nucleolus and nuclear matrix. Nucleoplasm plays a central role in genetic information flow and regulation of gene expression. Nuclear protein isolation has traditionally been accomplished by extraction protocol using detergent-containing buffers,..

Minute™ Plasma Membrane Protein Isolation and Cell Fractionation Kit (50 Preps) (Invent Biotechnologies, Inc.)

The MinuteTM kit represents the next generation product for plasma membrane (PM) isolation and cell fractionation, offering unsurpassed convenience and consistency by eliminating uncontrollable variations associated with traditional homogenization and density gradient centrifugation including two phase partitioning.

Minute™ Cytoplasmic & Nuclear Extraction Kits for Cells (50 Preps) (Invent Biotechnologies, Inc.)

Minute™ Cytoplasmic and Nuclear Extraction Kit for Cells is composed of optimized cytoplasmic extraction buffer, nuclear protein extraction buffer and protein extraction filter cartridges with 2.0 ml collection tubes.