SPIN-COLUMN-BASED PROTEIN EXTRACTION / CELL FRACTIONATION

Founded in 2010, Invent Biotechnologies is recognized worldwide for having revolutionized protein extraction and cell fractionation. As the inventor of a range of spin column-based disruptive technologies for protein research, we offer a simpler, smarter approach to sample preparation. This translates to streamlined workflows and more consistent results, providing superior data quality. By choosing our Minute™ Kits for protein extraction and cell fractionation, researchers can be confident that their samples accurately represent the endogenous profile of the cells or tissue under investigation. Requiring only a benchtop centrifuge, Minute™ Kits minimize experimental variables and deliver uncompromised material for more reliable data. The success of the Minute™ approach is evidenced by a growing number of publications, including citations in Nature, Science and Cell.

   

Minute™ Plant Plasma Membrane Protein Isolation Kit (50 Preps)    (Invent Biotechnologies, Inc.) Plasma membrane (PM) protein accounts for a small fraction of total cellular protein in plants but performs a very critical role in plant physiology. Isolation and purification of PM protein from plant tissues have been traditionally done by sucrose density ultracentrifugation and aqueous two-phase partitioning.

Minute™ High-Efficiency Saliva Exosome Isolation Kit (50 Preps)    (Invent Biotechnologies, Inc.) Diagnoses using exosomes derived from saliva have been attracting great attention in recent years due to the ease of sample collection. However, saliva samples are challenging when it comes down to exosome isolation. In addition to cells and cell debris..

Minute™ High-Efficiency Exosome Precipitation Reagent (20 ml)    (Invent Biotechnologies, Inc.) Exosomes are vesicles secreted by cells. They are present in a variety of body fluids such as serum, ascites, spinal cord fluid, urine, and saliva. Cultured cells also secrete significant number of exosomes. The size distribution of exosome is ranging from 30-120 nm.

Minute™ Yeast Mitochondria Enrichment Kit (50 Preps)    (Invent Biotechnologies, Inc.) Traditional protocol for yeast mitochondria isolation/extraction involves a range of centrifugation-based sub-cellular fractionation procedures. Typically, the techniques include spheroplast preparation, glass-bead lysis using a homogenization instrument, ..

Minute™ Mitochondria Isolation Kit for Mammalian Cells and Tissues (50 Preps)    (Invent Biotechnologies, Inc.) Minute™ Mitochondria Isolation Kit for Mammalian Cells and Tissues is composed of optimized buffers and protein extraction filter cartridges with 2.0 ml collection tubes. The kit is designed to rapidly isolate native mitochondria proteins from cultured mammalian cells or tissues.

Minute™ Lysosome Isolation Kit for Mammalian Cells/Tissues (20 Preps)    (Invent Biotechnologies, Inc.) Lysosomes are spherical vesicles in eukaryotic cells that are responsible for waste removal. The digestive enzymes contained in lysosomes play a vital role in digesting excess or worn-out organelles, food particles and engulfed viruses or bacteria. Lysosomes are relatively large organelles ranging in size from 0.1 to 1.2 um.

Minute™ Golgi Apparatus Enrichment Kit (20 Preps)    (Invent Biotechnologies, Inc.) Golgi apparatus, also called Golgi complex or Golgi body, is made up of a series of flattened stacked pouches (cisternae). Golgi apparatus is a vital organelle in eukaryotic cells and is responsible for transporting, modifying, and packaging protein and lipids into vesicles for delivery to targeted locations.

Minute™ High Fidelity ER Isolation Kit for Liver Tissue (20 Preps)    (Invent Biotechnologies, Inc.) Endoplasmic reticulum (ER) is a major membranous structure that functionally connects nuclear membrane and plasma membrane. Liver is an ER-rich organ and has been used for isolation of ER for decades. Traditional methods for isolating ER are based on density-gradient ultracentrifugation.

Minute™ ER Enrichment Kit (20 Preps)    (Invent Biotechnologies, Inc.) Endoplasmic reticulum (ER) is a major membranous structure that functionally connects nuclear membrane and plasma membrane. ER plays a central role in exocytic pathway of protein trafficking in all eukaryotic cells. Proteins synthesized in cytoplasm are translocated into ER from where vesicles shuttle protein cargo to the Golgi apparatus..

Minute™ Endosome Isolation and Cell Fractionation Kit (20 Preps)    (Invent Biotechnologies, Inc.) Early endosomes, which come mainly from primary endocytic vesicles that fuse with each other, provide the starting point for late endosome maturation. In addition to their roles in normal cell physiology, endocytic processes play a key role in many diseases such as Alzheimer’s disease and inherited lysosomal storage diseases.