EZBlock™ Protease Inhibitor Cocktail VIII (BioVision Inc)
A cocktail of five protease inhibitors with broad specificity for the inhibition of aspartic, serine, cysteine, aminopeptidases and thermolysin-like proteases. This cocktail is recommended for purification of proteins containing His-Tag sequences. Provided in 1 ml DMSO. |
Protease Inhibitor Cocktails
Proteases are a family of naturally occurring regulatory enzymes that cleave the peptide bonds between the amino acids of polypeptide chains. They can be classified into six broad groups depending on the amino acid at which they cleave. These include Serine proteases, Threonine proteases, Cysteine proteases, Aspartate proteases, Metalloproteases, and Glutamic acid proteases. Based on the optimal pH of their activity, they are sub-classified into acid proteases, neutral proteases and alkaline proteases. Protease inhibitors are compounds that inhibit specific proteases (mentioned above). A delicate equilibrium of these proteases and their respective inhibitors can control proteolytic cascades, thereby regulating key events like coagulation, inflammation and acute phase response in the body.
During protein expression and isolation, endogenous cellular proteases can rapidly digest cellular proteins resulting in substantially diminished quality and quantity of protein samples needed for characterization and analysis. BioVision has introduced a new and exciting line of general, as well as specific protease inhibitors and cocktails, which can prevent in vitro proteolysis events. These protease inhibitors and cocktails are provided in an easy-to-use form and can be employed for inhibition of a broad range of proteases. The inhibitor cocktail kits are designed to include various combinations of inhibitors at their optimum concentrations, providing researchers with a number of options to specifically choose from, including the EDTA-free form.
Sample Preparation Kits
Cell Lysis Agents
Crosslinking Agents
Detergents
Dialysis Tubes
Phosphatase Inhibitor Cocktails
Protease Inhibitor Cocktails
Protein Enrichment Tools
Protein Labeling
Protein Purification
Protein Quantitation Kits
Spin Desalting Column
Tag Antibodies
Yeast Mitochondria
Protease Inhibitor Cocktails Products:
EZBlock™ Protease Inhibitor Cocktail VII (BioVision Inc)
A cocktail of six protease inhibitors with broad specificity for the inhibition of aspartic, serine, cysteine, aminopeptidases and metalloproteases. This cocktail is recommended for use with plant cell extracts. Each vial of the cocktail has the six inhibitors reconstituted in 1 ml DMSO |
EZBlock™ Protease Inhibitor Cocktail VI, General Use (BioVision Inc)
A cocktail of six protease inhibitors with broad specificity for the inhibition of serine, cysteine, aminopeptidases and metalloproteases. Recommended for general use. (Note: One vial is recommended for the inhibition of proteases present in 20 g of cell extract). Reconstitute each vial in 100 ml D.I. water to obtain 100 ml of the cocktail solution. |
EZBlock™ Protease Inhibitor Cocktail V, EDTA-Free (BioVision Inc)
A cocktail of four protease inhibitors that have been optimized for the inhibition of serine and cysteine but not metalloproteases. Since this cocktail does not contain any EDTA, metal-dependent proteins and IMAC |
EZBlock™ Protease Inhibitor Cocktail IX (BioVision Inc)
A cocktail of three protease inhibitors (shown in the table below for each vial) with specificity for the inhibition of cysteine proteases, including calpains, cathepsins and papain. Provided in 1 ml DMSO. Each vial contains 1.56 mM of ALLN (Cat. No. 1834), 500 µM of Antipain (Cat. No. 2834), and 1.5 mM of E-64 (Cat. No. 1739). The DMSO solution of the cocktail can be added to the aqueous buffer or directly to the extraction media. |
EZBlock™ Protease Inhibitor Cocktail IV (BioVision Inc)
A cocktail consisting of four broad spectrum protease inhibitors useful for the inhibition of aspartic, cysteine, serine, and metalloproteases. This protease inhibitor cocktail is recommended for use with fungal and yeast cell extracts. Recommended dilution: 1:100 -1:1000 (based on the applications) |
EZBlock™ Protease Inhibitor Cocktail III (BioVision Inc)
A cocktail of five broad specificity protease inhibitors (see table below) that can provide maximal protection to prevent degradation of proteins, especially in bacterial cell extracts (except metal ion chromatography applications) by inhibition of various aspartic, serine, cysteine, metalloproteases and aminopeptidases. |
EZBlock™ Protease Inhibitor Cocktail II, Animal-Free (BioVision Inc)
A cocktail consisting of five protease inhibitors useful for the inhibition of a broad range of proteases. Reconstitution of each vial with 1 ml DI water provides a 100X stock solution. Useful for the protection of proteins from endogenous proteases that can be released during protein extraction from cell lysates. The concentrations of the five protease inhibitors in a 1X solution are: AEBSF HCl (Cat. No. 1644, 500 µM); Aprotinin, Recombinant (Cat. No. 7866, 150 nM); E-64 (Cat. No. 1739, 1 µM), Leupeptin hemisulfate (Cat. No. 1648, 1 µM), and EDTA disodium (500 µM ). |
EZBlock™ Protease Inhibitor Cocktail II (BioVision Inc)
A cocktail consisting of five protease inhibitors useful for the inhibition of a broad range of proteases. Reconstitution of each vial with 1 ml DI water provides a 100X stock solution. Useful for the protection of proteins from endogenous proteases that can be released during protein extraction from cell lysates. |
EZblock™ Protease Inhibitor Cocktail (BioVision Inc)
A cocktail of four broad specificity protease inhibitors that can provide maximal protection to prevent degradation of proteins in mammalian tissue and cell extracts by inhibition of various serine and cysteine proteases. |