Streamlined chromatin immunoprecipitation procedure for investigating protein-DNA interactions.

Highlights

Robust immunoprecipitation and purification of DNA.
Unique workflow features a micro-elution (=6 µl) spin column for purification of ChIP DNA.
High-quality ChIP DNA is ideal for ChIP-qPCR, ChIP-Seq, and other molecular applications.

Description

The Zymo-Spin ChIP Kit from Zymo Research provides a streamlined ChIP procedure for investigating protein-DNA interactions that have been “fixed” in their natural state and can be used to effectively identify binding sites for transcription factors, co-factors, and other DNA regulatory proteins.

Technical Specifications

Applicable For	Chromatin Immunoprecipitation
Sample Source	Mammalian Cells
Storage	Upon arrival, ZymoMag Protein A should be stored at 4°C. All other components, including lyophilized Proteinase K, should be stored at room temperature. Once reconstituted, Proteinase K should be stored at ≤ -20&degC.

Citations

Reviews

“An excellent product that is easy to use.”

– A. A. – Karmanos Cancer Institute

“The kit reduced the amount of time needed for my ChIP experiments, the DNA purification was more efficient than other kits, and the purity of the DNA was higher. The endpoint results generated were highly informative to my experimental objectives.”

– F. E. – Cleveland Clinic

Kit Components

Cat #	Name	Size
D3004-4-10	DNA Elution Buffer	10 ml
D3004-4-16	DNA Elution Buffer	16 ml
D4003-2-6	DNA Wash Buffer (Concentrate)	6 ml
D5201-1-50	ChIP DNA Binding Buffer	50 ml
D5210-1-30	Chromatin Shearing Buffer	30 ml
D5210-2-30	Chromatin Dilution Buffer	30 ml
D5210-3-30	Chromatin Wash Buffer I	30 ml
D5210-4-30	Chromatin Wash Buffer II	30 ml
D5210-5-30	Chromatin Wash Buffer III	30 ml
D5210-6-10	5X Chromatin Elution Buffer	10 ml
D5210-7-1	5M NaCl	1 ml
D5220-2-30	Nuclei Prep Buffer	30 ml
M2001	ZymoMag Protein A	200 µl
M2001-2	ZymoMag Protein A	400 µl

Zymo-Spin ChIP Kit

D5209 / D5210

Cat #	Name	Size
D5209	Zymo-Spin ChIP Kit	10 Preps
D5210	Zymo-Spin ChIP Kit	25 Preps

Documents

Protocol:

Datasheet:

SDS (MSDS): D5209

Quick Protocol D5210