Control for bisulfite conversion; DNAmethylation quantitation

Highlights

Purified, non-methylated and methylated human DNA for use as negative and positive control in methylation detection applications.
Standards can be assayed in parallel with samples to monitor bisulfite conversion efficiency.
Ideal controls for bisulfite sequencing PCR (BSP) and methylation specific PCR (MSP). Primers included with the full set are designed to amplify non-methylated, methylated, and mixed methylated copies of the Ras association (RalGDS/AF-6) domain family member 1 (RASSF1) gene following bisulfite conversion.

Description

The Human WGA Methylated & Non-methylated DNA Set consists of two control DNAs (non-methylated and methylated) along with a set of specifically designed primers that can be used in conjunction with the EZ DNA Methylation, EZ DNA Methylation-Gold, EZ DNA Methylation-Direct, and EZ DNA Methylation-Lightning kits from Zymo Research to assess the efficiency of bisulfite-mediated conversion of DNA. The Human WGA Methylated & Non-methylated DNA Set is generated using phi29 DNA polymerase based whole genome amplification techniques from HCT116 DKO cell line derived genomic DNA (Human HCT116 DKO Non-methylated DNA). The Human WGA Methylated DNA is Human WGA Non-methylated DNA that has been enzymatically methylated at all double-stranded CG dinucleotides using M.SssI methyltransferase and can be used as a positive control for DNA methylation analysis.

Technical Specifications

Concentration	250 ng/µl in buffer (10 mM Tris-HCl, 1 mM EDTA, pH 8.0)
Sample Source	Whole genome amplified DNA from HCT116 DKO cells. Human WGA Methylated DNA is enzymatically methylated by M.SssI methyltransferase.
Sample Storage	≤ -20°C

Product FAQ

Q1: Are the Human methylated DNA standards completely (100%) methylated at the CpG sites?

All standards are enzymatically methylated by CpG Methylase in vitro at all CpG dinucleotides. The methylated DNA is very highly methylated (> 95% of all CpG sites).

Citations

Human Methylated & Non-Methylated (WGA) DNA Set

D5013 / D5013-1 / D5013-2

Cat #	Name	Size
D5013	Human Methylated & Non-Methylated (WGA) DNA Set	5 µg/20 µl
D5013-1	Human WGA Non-Methylated DNA	5 µg/20 µl
D5013-2	Human WGA Methylated DNA	5 µg/20 µl

Documents

Protocol:

Datasheet:

SDS (MSDS):