- Control DNA for 5-methylcytosine and 5-hydroxymethylcytosine quantitation applications (ex. mass spectrometry, HPLC, TLC, etc.)
- Substrate for studies involving methylated cytosine and interacting proteins
The 5-Methylcytosine & 5-Hydroxymethylcytosine DNA Standard Set is a set of three standards that are linear dsDNA at a length of 897 bp. All DNA standards have the same sequence (see protocol for sequence), the only difference being that each standard contains either 100% unmodified cytosines, 5-methylcytosines, or 5-hydroxymethylcytosines. Since the sequence and extent of cytosine modification is known, this DNA standard set is ideal for use in calibration of various applications intended for quantitation of cytosine modifications.
|Applicable For||Cytosine modification studies (i.e. 5-mC & 5-hmC), mass spectrometry, HPLC, TLC, etc.|
|Concentration||50 ng/µl each in 40 µl volume|
|Sample Storage||Store reagents at -20°C for up to 12 months, Prolonged storage should be ≤ -70°C|
5-Methylcytosine & 5-Hydroxymethylcytosine DNA Standard Set