Highlights

Highly processive enzyme for specific modification of 5-hydroxymethylcytosine (5-hmC) with a glucose moiety.
Ideal for locus specific and global quantification of hydroxymethylated DNA.

Description

The 5-hmC Glucosyltransferase is a highly active enzyme that specifically tags 5-hydroxymethylcytosine in DNA with a
glucose moiety to yield glucosyl-5-hydroxymethylcytosine, which in turn can be used for sequence-specific, genome-wide, or global 5-hmC detection.

Technical Specifications

Concentration	2 U/µl
Standard Reaction Time	2 hours
Storage	5-hmC Glucosyltransferase is guaranteed for 12 months at -20°C. Avoid multiple freeze/thaw cycles. For long-term storage, aliquoting is recommended with storage at -80°C.
Unit Definition	One unit (U) is defined as the amount of enzyme needed to protect 1 µg of 5-hmC DNA Standard [D5405-3] from Csp6I restriction enzyme digestion via glucosylation in a reaction incubated at 30°C for 1 hour.

Citations

5-hmC Glucosyltransferase

E2026 / E2027

Cat #	Name	Size
E2026	5-hmC Glucosyltransferase	100 U
E2027	5-hmC Glucosyltransferase	200 U

Documents

Protocol:

SDS (MSDS):