- Highly processive enzyme for specific modification of 5-hydroxymethylcytosine (5-hmC) with a glucose moiety.
- Ideal for locus specific and global quantification of hydroxymethylated DNA.
The 5-hmC Glucosyltransferase is a highly active enzyme that specifically tags 5-hydroxymethylcytosine in DNA with a
glucose moiety to yield glucosyl-5-hydroxymethylcytosine, which in turn can be used for sequence-specific, genome-wide, or global 5-hmC detection.
|Standard Reaction Time||2 hours|
|Storage||5-hmC Glucosyltransferase is guaranteed for 12 months at -20°C. Avoid multiple freeze/thaw cycles. For long-term storage, aliquoting is recommended with storage at -80°C.|
|Unit Definition||One unit (U) is defined as the amount of enzyme needed to protect 1 µg of 5-hmC DNA Standard [D5405-3] from Csp6I restriction enzyme digestion via glucosylation in a reaction incubated at 30°C for 1 hour.|