5-Methylcytosine & 5-Hydroxymethylcytosine DNA Standard Set

5-Methylcytosine & 5-Hydroxymethylcytosine DNA Standard Set

Highlights

  • Control DNA for 5-methylcytosine and 5-hydroxymethylcytosine quantitation applications (ex. mass spectrometry, HPLC, TLC, etc.)
  • Substrate for studies involving methylated cytosine and interacting proteins


Description

The 5-Methylcytosine & 5-Hydroxymethylcytosine DNA Standard Set is a set of three standards that are linear dsDNA at a length of 897 bp. All DNA standards have the same sequence (see protocol for sequence), the only difference being that each standard contains either 100% unmodified cytosines, 5-methylcytosines, or 5-hydroxymethylcytosines. Since the sequence and extent of cytosine modification is known, this DNA standard set is ideal for use in calibration of various applications intended for quantitation of cytosine modifications.

Technical Specifications

Applicable For Cytosine modification studies (i.e. 5-mC & 5-hmC), mass spectrometry, HPLC, TLC, etc.
Concentration 50 ng/µl each in 40 µl volume
Sample Storage Store reagents at -20°C for up to 12 months, Prolonged storage should be ≤ -70°C

Citations

Kit Components

5-Methylcytosine & 5-Hydroxymethylcytosine DNA Standard Set

D5405

Cat # Name Size
D5405 5-Methylcytosine & 5-Hydroxymethylcytosine DNA Standard Set Full Set

Documents

Protocol: 
SDS (MSDS):