ZymoPURE Plasmid Kits

ZymoPURE Plasmid Kits

ZymoPURE II Plasmid kits are the fastest and simplest plasmid kits available to efficiently isolate transfection-grade plasmid DNA from E. coli (up to 400 µg in 18 minutes). From these plasmid kits, the eluted plasmid DNA is ready for immediate use, without any alcohol precipitation. These plasmid kits feature ZymoPURE II technology, including a modified alkaline lysis system and novel binding chemistry. Together, these plasmid kits yield highly concentrated plasmid DNA (up to 3 µg/µl) directly from a spin column.

Instead of time-consuming gravity flow columns, these plasmid kits utilize vacuum filtration and binding for rapid sample processing. As an added convenience, these plasmid kits contain colored buffers that permit error-free visualization and identification of complete bacterial cell lysis and neutralization. The wash regimen of these plasmid kits has been optimized to ensure the eluted DNA is free of endotoxins, salt, protein, and RNA resulting in plasmids that are suitable for sensitive applications including transfection, plasmid-mediated gene silencing, cloning, and sequencing. Finally, the specially designed spin-columns in these plasmid kits allow for elutions on a microcentrifuge.

*Patent Pending

Comparison Table

Catalog # Product Binding Capacity Elution Volume Culture Input Endotoxin Levels Format
D4208T ZymoPURE Plasmid Miniprep 100 μg ≥ 25 μl ≤ 5 mL Culture ≤ 1 EU/μg Spin Column
D4202 ZymoPURE II Plasmid Maxiprep 1.2 mg ≥ 200 μl ≤ 150 ml ≤ 0.025 EU/μg DNA Spin Column
D4200 ZymoPURE II Plasmid Midiprep 400 μg ≥ 100 μl ≤ 50 ml ≤ 0.025 EU/μg DNA Spin Column
D4204 ZymoPURE II Plasmid Gigaprep 10 mg ≥ 2 ml ≤ 2.5 L ≤ 0.025 EU/μg DNA Spin Column
D4213 ZymoPURE-Express Plasmid Midiprep 1.2 mg ≥ 200 μl 25 mL ≤ 1 EU/μg Plasmid DNA Spin Column