ZymoPURE Plasmid Kits
ZymoPURE II Plasmid kits are the fastest and simplest plasmid kits available to efficiently isolate transfection-grade plasmid DNA from E. coli (up to 400 µg in 18 minutes). From these plasmid kits, the eluted plasmid DNA is ready for immediate use, without any alcohol precipitation. These plasmid kits feature ZymoPURE II technology, including a modified alkaline lysis system and novel binding chemistry. Together, these plasmid kits yield highly concentrated plasmid DNA (up to 3 µg/µl) directly from a spin column.
Instead of time-consuming gravity flow columns, these plasmid kits utilize vacuum filtration and binding for rapid sample processing. As an added convenience, these plasmid kits contain colored buffers that permit error-free visualization and identification of complete bacterial cell lysis and neutralization. The wash regimen of these plasmid kits has been optimized to ensure the eluted DNA is free of endotoxins, salt, protein, and RNA resulting in plasmids that are suitable for sensitive applications including transfection, plasmid-mediated gene silencing, cloning, and sequencing. Finally, the specially designed spin-columns in these plasmid kits allow for elutions on a microcentrifuge.
|Catalog #||Product||Binding Capacity||Elution Volume||Culture Input||Endotoxin Levels||Format|
|D4208T||ZymoPURE Plasmid Miniprep||100 μg||≥ 25 μl||≤ 5 mL Culture||≤ 1 EU/μg||Spin Column|
|D4202||ZymoPURE II Plasmid Maxiprep||1.2 mg||≥ 200 μl||≤ 150 ml||≤ 0.025 EU/μg DNA||Spin Column|
|D4200||ZymoPURE II Plasmid Midiprep||400 μg||≥ 100 μl||≤ 50 ml||≤ 0.025 EU/μg DNA||Spin Column|
|D4204||ZymoPURE II Plasmid Gigaprep||10 mg||≥ 2 ml||≤ 2.5 L||≤ 0.025 EU/μg DNA||Spin Column|
|D4213||ZymoPURE-Express Plasmid Midiprep||1.2 mg||≥ 200 μl||25 mL||≤ 1 EU/μg Plasmid DNA||Spin Column|