ZymoPURE Plasmid Kits

ZymoPURE Plasmid kits are the fastest and simplest plasmid kits available to efficiently isolate transfection-grade plasmid DNA from E. coli (up to 3 mg in 16 minutes). These plasmid kits feature our advanced ZymoPURE purification technology, including a modified alkaline lysis system and patented binding chemistry, enabling the recovery of highly concentrated plasmid DNA (up to 6 µg/µl) directly from a spin column using microcentrifuge.

Instead of time-consuming gravity flow columns found in conventional transfection-grade plasmid kits, these plasmid kits utilize vacuum filtration and binding for rapid sample processing. Furthermore, the eluted plasmid DNA is ready for immediate use, without the need for alcohol precipitation. Resulting in the purification of transfection-grade plasmid DNA up to 9 times faster then traditional methods.

As an added convenience, these plasmid kits contain colored buffers that permit error-free visualization and identification of complete bacterial cell lysis and neutralization. The wash regimen of these plasmid kits has been optimized to ensure the eluted DNA is free of endotoxins, salt, protein, and RNA resulting in plasmids that are suitable for sensitive applications including transfection, in vivo injections, gene editing, recombinant viral vector production, PCR and sequencing. 

COMPARISON TABLE

Catalog #ProductBinding CapacityElution VolumeCulture InputEndotoxin LevelsFormat
D4208TZymoPURE Plasmid Miniprep100 µg≥ 25 µl≤ 5 mL Culture≤ 1 EU/µg DNASpin-Column
D4214ZymoPURE 96 Plasmid Miniprep Kinew!100 μg≥ 125 µL≤ 5 mL Culture≤ 1 EU/µg DNA96-Well Plate
D4200ZymoPURE II Plasmid Midiprep1.2 mg≥ 150 µl≤ 50 ml≤ 0.025 EU/µg DNASpin-Column
D4202ZymoPURE II Plasmid Maxiprep3.0 mg≥ 300 µl≤ 150 ml≤ 0.025 EU/µg DNASpin-Column
D4204ZymoPURE II Plasmid Gigaprep25mg≥ 3 ml≤ 2.5 L≤ 0.025 EU/µg DNASpin-Column
D4213ZymoPURE-Express Plasmid Midiprep1.2 mg≥ 150 µl25 mL≤ 1 EU/µg Plasmid DNASpin-Column

D4208T / D4209 / D4210 / D4211 / D4212


Part of the ZymoPURE™ Plasmid Kits collection, the ZymoPURE™ Plasmid Miniprep Kit features a spin column-based method for the purification of up to 100 µg of ultra-pure endotoxin-free plasmid DNA in less than 15 minutes. The unique spin-column design also provides zero buffer…

D4214 / D4215

The ZymoPURE™ 96 Plasmid Miniprep Kit features a high-throughput method for the purification of up to 100 µg of ultra-pure transfection-grade plasmid DNA using a vacuum manifold or centrifuge. The unique vacuum plate design provides zero buffer retention and low elution volumes….

D4200 / D4201

Part of the ZymoPURE Plasmid Kits collection, the ZymoPURE II Plasmid Midiprep kit provides the fastest and simplest method available to efficiently isolate up to 1.2 mg of transfection grade plasmid DNA from E….

D4202 / D4203

Part of the ZymoPURE Plasmid Kits collection, the ZymoPURE II Plasmid Maxiprep kit provides the fastest and simplest method available to efficiently isolate up to 3 mg of transfection grade plasmid DNA from E. coli. Utilizing…

D4204

Part of the ZymoPURE Plasmid Kits collection, the ZymoPURE II Plasmid Gigaprep kit provides the fastest and simplest method available to efficiently isolate up to 25 mg of transfection grade plasmid DNA from E. coli. Utilizing…

D4213

Part of the ZymoPURE Plasmid Kits collection, the ZymoPURE – Express Plasmid Midiprep kit utilizes a patented alkaline lysis method for purifying up to 1.2 mg of transfection-grade plasmid DNA directly from E….