ZymoBIOMICS DNA Microprep Kit

ZymoBIOMICS DNA Microprep Kit

Highlights

  • Validated Unbiased for Microbiome Measurements: Unbiased cellular lysis validated using the ZymoBIOMICS Microbial Community Standard.
  • Inhibitor-Free DNA from Any Sample: Isolate ultra-pure DNA ready for any downstream application.
  • Certified Low Bioburden: Boost your detection limit for low abundance microbes.


Description

The ZymoBIOMICS DNA Kits are microbial DNA purification kits designed for purifying DNA from a variety of sample inputs that is immediately ready for microbiome or metagenome analyses. The ZymoBIOMICS lysis system eliminates bias associated with unequal lysis efficiencies of different organisms (e.g. Gram-negative/positive bacteria, fungus, protozoans, and algae) making it ideal for microbial community profiling. Uniform mechanical lysis of tough microbes is achieved by bead beating with the innovative ultra-high density BashingBeads. This kit is equipped with our OneStep PCR Inhibitor removal technology, enabling PCR amplification from DNA derived from inhibitor-rich environmental samples. Purified DNA is ideal for all downstream applications including PCR, arrays, 16S rRNA gene sequencing, and shotgun sequencing. DNA Size is 15-20 kb.

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Technical Specifications

Applicable For All sensitive downstream applications such as qPCR and Next-Generation Sequencing.
Elution Volume = 10 µl ZymoBIOMICS DNase/RNase Free Water
Equipment Microcentrifuge, vortex/Disruptor Genie, high speed cell disruptor (recommended).
Processing Volume Fecal =50 mg, Soil = 100 mg, Cells = 20 mg (Approximately equal to 2×108 bacterial, 2×107 yeast, or 2×106 mammalian cells)
Purity High quality, inhibitor-free DNA. Typical A260/A280 =1.8
Sample Source Bacterial, fungal, protozoan, algae, viral, mitochondrial, and host DNA is efficiently isolated from feces, soil, fungal/bacterial cells, biofilms and water.
Sample Storage Eluted DNA should be stored at = -20°C.
Size Range Typically 15-20 kb post-bead beating. For optimal DNA integrity, collect samples in DNA/RNA Shield.
Type Total DNA
Yield Up to 5 µg total DNA can be eluted into = 10 µl

Citations

Kit Components

ZymoBIOMICS DNA Microprep Kit

Cat # Name Size
D4301 ZymoBIOMICS DNA Microprep Kit (Lysis Tubes) 50 Preps
D4305 ZymoBIOMICS DNA Microprep Kit (No Lysis Matrix) 50 Preps

Documents

Protocol: 
Datasheet: 
SDS (MSDS): D4301   D4305  
Optimized Lysis Protocols