Simple: Fast spin-column procedure yields pure yeast RNA without using glass beads or phenol.
Versatile: Efficient RNA isolation from a broad spectrum of fungal species susceptible to Zymolyase.
High-Quality: Isolated RNA is suitable for use in RT-PCR, Northern blotting, etc.
Description
The YeaStar RNA Kit provides all the necessary reagents for RNA isolation from a broad spectrum of fungi including: Aspergillus fumigatus, Aspergillus nidulans, Aspergillus nivens var. aureus, Candida albicans/, Pichia pastoris, Saccharomyces cerevisiae, Schizosaccharomyces pombe. Generally, the kit can be used for the purification of high-quality, total RNA from any fungus that can be lysed by yeast lytic enzyme. The kit facilitates the purification of 10-25 µg of total RNA from 1-1.5 ml of cultured cells using innovative Zymo-Spin column technology.
Technical Specifications
Applicable For
Isolated RNA is well suited for downstream applications such as Northern blotting, RT-PCR, Next-Generation Sequencing, Microarray, hybridization and other sensitive applications
Elution Volume
≥ 60 µl DNase/RNase-Free Water
Equipment
Microcentrifuge & Heat Block/Bath
Processing Time
30 minutes
Processing Volume
≤ 1.5 ml of Culture (1-5 x 107 cells)
Purity
Typical A260/A280 & A260/A230 ≥ 1.8
Sample Source
Cell Culture (Colonies/Patches or Liquid Culture)
Size Range
≥ 17 nt
Type
Total RNA
Yield
≤ 25 µg of total RNA
Product FAQ
Q1: What strains is this kit compatible with?
Any strains susceptible to Zymolyase, which includes the following fungal genera:
