- NGS-Ready: RNA is ready for all downstream applications including Next-Gen Sequencing, RT-qPCR, hybridization, etc.
- Ultra-Pure: Eliminate contaminants and inhibitors in 5 minutes.
- Maximum Recovery: Recover >90% and elute in as little as 6 µl.
|Purity||RNA is ready for Next-Gen sequencing, RT-PCR, microarray, hybridization, etc. A260/A280, A260/A230: > 1.8.|
|Sample Source||DNase I treated RNA, in vitro transcription products, the aqueous phase following TRIzol/chloroform or similar extraction|
|Size Range||Total RNA ≥ 17 nt|
|Yield||10 µg RNA (binding capacity), ≥ 6 µl (elution volume)|
Q1: Can the RCC kit be used to purify DNA (cDNA)?
Q2: Should carrier RNA be added to the sample prior to purification?
Q3: Will this kit remove fluorescent dyes, radiolabeled dNTP’s and/or Biotin?
Q4: Are samples containing high concentration of detergents, salts, formamide or sucrose compatible with the RCC buffer system?
Q5: Can other DNase I enzymes or sets (e.g. Qiagen DNase I) be used?
Q6: Is DNase I treatment necessary?
“It isolates small RNAs in the same go, unlike MinElute columns which leaves out RNAs smaller than 200nt.”
– Anupma C.
“The Zymo-Spin IC column is convenient.”
– Lihua D. (National Institutes of Health)
“Kit was easy and fast, and cleaned up my RNA samples very well. It did exactly what I needed it to do.”
– Caroline S. (Cornell University)
“Great for purifying small amounts of RNA. I use a bioanalyzer after and I can see the difference from before, cleaner bands and the concentration increases with a smaller amount of elutant.”
– Catherine A. (USAMRIID)
“The product is very easy to use, very quick protocol for an excellent RNA clean up. It also purifies small RNA which is not the case of all RNA clean up columns.”
– Agnes N. (Biofidal)
“Zymo RNA Clean & Concentrator is the best product for CRISPR applications. We have tried several other products including precipitation methods, Zymo product gave us the best results and yield.”
– Gaurav V. (OMRF)
“Great result. Everybody should try this product. I’ve never seen such purity of the RNA before. This product works excellent and easy to use and affordable.”
– Para S. (University of Louisville)
“I used this as a clean-up step after guanidinium thiocyanate-phenol-chloroform extraction from homogenized tissues. I found the process very simple, and adding the kit on did seem to significantly improve the quality of the RNA pool relative to the original phenol-chloroform extraction.”
– Emily G. (University of Wisconsin-Madison)
“It’s [an] excellent kit to work with tissues with high poly-phenols and acids.”
– Arun D. (University of Missouri)
“Great yield from a tiny amount of genomic RNA.”
– Andrew H. (University of British Columbia)
RNA Clean & Concentrator-5